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The POX1 gene encoding peroxisomal acyl-CoA oxidase in Saccharomyces cerevisiae is under the control of multiple regulatory elements
Transcription of POX1, the gene encoding peroxisomal acyl-CoA oxidase in the yeast Saccharomyces cerevisiae, is controlled by the carbon source given for cell growth. The gene is repressed in glucose, derepressed in glycerol, and induced in oleate. This regulation is controlled by cis-acting element...
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| Published in: | The Journal of biological chemistry 1994-09, Vol.269 (39), p.24480-24485 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c430t-bf8f26ca5a9b172ebc05bf63b5ec2c63621b936552491d55df7cbbccbdff9b553 |
| container_end_page | 24485 |
| container_issue | 39 |
| container_start_page | 24480 |
| container_title | The Journal of biological chemistry |
| container_volume | 269 |
| creator | Wang, T. (Mount Sinai School of Medicine, New York, NY.) Luo, Y Small, G.M |
| description | Transcription of POX1, the gene encoding peroxisomal acyl-CoA oxidase in the yeast Saccharomyces cerevisiae, is controlled by the carbon source given for cell growth. The gene is repressed in glucose, derepressed in glycerol, and induced in oleate. This regulation is controlled by cis-acting elements in the 5' region of the gene, which bind regulatory proteins. By deletion analysis and DNA band shift assays, we have characterized three such elements in the POX1 upstream sequence, two upstream repression sequences and an upstream activating sequence. Each was able to regulate the transcription of a heterologous gene construct iso-1-cytochrome-c (CYC1)/lacZ |
| doi_str_mv | 10.1016/S0021-9258(19)51109-7 |
| format | article |
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(Mount Sinai School of Medicine, New York, NY.) ; Luo, Y ; Small, G.M</creator><creatorcontrib>Wang, T. (Mount Sinai School of Medicine, New York, NY.) ; Luo, Y ; Small, G.M</creatorcontrib><description>Transcription of POX1, the gene encoding peroxisomal acyl-CoA oxidase in the yeast Saccharomyces cerevisiae, is controlled by the carbon source given for cell growth. The gene is repressed in glucose, derepressed in glycerol, and induced in oleate. This regulation is controlled by cis-acting elements in the 5' region of the gene, which bind regulatory proteins. By deletion analysis and DNA band shift assays, we have characterized three such elements in the POX1 upstream sequence, two upstream repression sequences and an upstream activating sequence. 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(Mount Sinai School of Medicine, New York, NY.)</creatorcontrib><creatorcontrib>Luo, Y</creatorcontrib><creatorcontrib>Small, G.M</creatorcontrib><title>The POX1 gene encoding peroxisomal acyl-CoA oxidase in Saccharomyces cerevisiae is under the control of multiple regulatory elements</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Transcription of POX1, the gene encoding peroxisomal acyl-CoA oxidase in the yeast Saccharomyces cerevisiae, is controlled by the carbon source given for cell growth. The gene is repressed in glucose, derepressed in glycerol, and induced in oleate. This regulation is controlled by cis-acting elements in the 5' region of the gene, which bind regulatory proteins. By deletion analysis and DNA band shift assays, we have characterized three such elements in the POX1 upstream sequence, two upstream repression sequences and an upstream activating sequence. Each was able to regulate the transcription of a heterologous gene construct iso-1-cytochrome-c (CYC1)/lacZ</description><subject>Acyl-CoA Oxidase</subject><subject>Base Sequence</subject><subject>Coatomer Protein</subject><subject>DNA, Fungal</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>GENE</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Gene Expression Regulation, Fungal</subject><subject>GENES</subject><subject>Genes, Fungal</subject><subject>GENETICA</subject><subject>GENETIQUE</subject><subject>Microbodies - enzymology</subject><subject>Molecular Sequence Data</subject><subject>Oleic Acid</subject><subject>Oleic Acids - metabolism</subject><subject>ORGANITE CELLULAIRE</subject><subject>ORGANULOS CITOPLASMICOS</subject><subject>Oxidoreductases - genetics</subject><subject>OXIDORREDUCTASAS</subject><subject>OXYDOREDUCTASE</subject><subject>Promoter Regions, Genetic</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNqFkcFq3DAQhkVpSDdpX6AQ0KGU9OBWki3ZOoYlaQuBFDaB3IQkj2wV2dpKdtu998HrzS65Zi6C-b-ZQXwIXVDymRIqvmwIYbSQjDeXVH7ilBJZ1K_QipKmLEpOH1-j1TPyBp3l_JMsVUl6ik5rySSlbIX-3feAf9w9UtzBCBhGG1s_dngLKf71OQ46YG13oVjHK7x0Wp0B-xFvtLW9TnHYWcjYQoLfPnu9ZBnPYwsJT8tiG8cpxYCjw8McJr8NgBN0c9BTTDsMAQYYp_wWnTgdMrw7vufo4eb6fv2tuL37-n19dVvYqiRTYVzjmLCaa2lozcBYwo0TpeFgmRWlYNTIUnDOlk-2nLeutsZYa1rnpOG8PEcfD3u3Kf6aIU9q8NlCCHqEOGdVi7qSDX0ZpELwRlZ7kB9Am2LOCZzaJj_otFOUqL0m9aRJ7R0oKtWTJlUvcxfHA7MZoH2eOnpZ8g-HvPdd_8cnUMZH28OgmJCqlIpVVUMW7P0Bczoq3SWf1cNGcs4Fq8v_QvCkog</recordid><startdate>19940930</startdate><enddate>19940930</enddate><creator>Wang, T. 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(Mount Sinai School of Medicine, New York, NY.) ; Luo, Y ; Small, G.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-bf8f26ca5a9b172ebc05bf63b5ec2c63621b936552491d55df7cbbccbdff9b553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Acyl-CoA Oxidase</topic><topic>Base Sequence</topic><topic>Coatomer Protein</topic><topic>DNA, Fungal</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>GENE</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Gene Expression Regulation, Fungal</topic><topic>GENES</topic><topic>Genes, Fungal</topic><topic>GENETICA</topic><topic>GENETIQUE</topic><topic>Microbodies - enzymology</topic><topic>Molecular Sequence Data</topic><topic>Oleic Acid</topic><topic>Oleic Acids - metabolism</topic><topic>ORGANITE CELLULAIRE</topic><topic>ORGANULOS CITOPLASMICOS</topic><topic>Oxidoreductases - genetics</topic><topic>OXIDORREDUCTASAS</topic><topic>OXYDOREDUCTASE</topic><topic>Promoter Regions, Genetic</topic><topic>SACCHAROMYCES CEREVISIAE</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, T. 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(Mount Sinai School of Medicine, New York, NY.)</au><au>Luo, Y</au><au>Small, G.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The POX1 gene encoding peroxisomal acyl-CoA oxidase in Saccharomyces cerevisiae is under the control of multiple regulatory elements</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1994-09-30</date><risdate>1994</risdate><volume>269</volume><issue>39</issue><spage>24480</spage><epage>24485</epage><pages>24480-24485</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Transcription of POX1, the gene encoding peroxisomal acyl-CoA oxidase in the yeast Saccharomyces cerevisiae, is controlled by the carbon source given for cell growth. The gene is repressed in glucose, derepressed in glycerol, and induced in oleate. This regulation is controlled by cis-acting elements in the 5' region of the gene, which bind regulatory proteins. 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| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1994-09, Vol.269 (39), p.24480-24485 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76749815 |
| source | ScienceDirect® |
| subjects | Acyl-CoA Oxidase Base Sequence Coatomer Protein DNA, Fungal Fungal Proteins - genetics Fungal Proteins - metabolism GENE Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal GENES Genes, Fungal GENETICA GENETIQUE Microbodies - enzymology Molecular Sequence Data Oleic Acid Oleic Acids - metabolism ORGANITE CELLULAIRE ORGANULOS CITOPLASMICOS Oxidoreductases - genetics OXIDORREDUCTASAS OXYDOREDUCTASE Promoter Regions, Genetic SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins |
| title | The POX1 gene encoding peroxisomal acyl-CoA oxidase in Saccharomyces cerevisiae is under the control of multiple regulatory elements |
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