Effect of Activated Lymphocytes on the Regulation of Hematopoiesis: Enhancement and Suppression of In Vitro BFU-E Growth by T Cells Stimulated by Autologous Non-T Cells

Autologous mixed lymphocyte culture (AMLR) is an immunologic response with memory and specificity and plays a role in immune regulation. Effects of T cells activated by AMLR were studied in the regulation of in vitro erythropoiesis. AMLR-activated T cells were cocultured with autologous non-T, nonph...

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Bibliographic Details
Published in:Blood 1986-04, Vol.67 (4), p.1143-1147
Main Authors: Harada, Mine, Nakao, Shinji, Kondo, Kunio, Odaka, Kazuaki, Ueda, Mikio, Shiobara, Shintaro, Matsue, Kosei, Mori, Takao, Matsuda, Tamotsu
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
Antigens, Differentiation, T-Lymphocyte
Antigens, Surface - immunology
Biological and medical sciences
Cell Division
Cell interactions, adhesion
Colony-Forming Units Assay
Erythrocytes - cytology
Erythropoiesis
Fundamental and applied biological sciences. Psychology
Hematopoietic Stem Cells - cytology
Histocompatibility Antigens Class II - analysis
Humans
Lymphocyte Activation
Lymphocyte Culture Test, Mixed
Molecular and cellular biology
Phenotype
T-Lymphocytes - classification
T-Lymphocytes - immunology
T-Lymphocytes - physiology
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Summary:Autologous mixed lymphocyte culture (AMLR) is an immunologic response with memory and specificity and plays a role in immune regulation. Effects of T cells activated by AMLR were studied in the regulation of in vitro erythropoiesis. AMLR-activated T cells were cocultured with autologous non-T, nonphagocytic peripheral blood mononuclear cells for assaying erythroid progenitor cells (BFU-E). T cells activated for 3 days in AMLR showed significant enhancement of in vitro colony growth by BFU-E. In contrast, activated T cells from day 7 AMLR caused significant suppression of BFU-E growth. Both enhancing and suppressing activities of AMLR-activated T cells were mediated by an la-positive and radiosensitive population within the OKT4+ subset. These observations suggest that AMLR-activated T cells may play a role in the immune-mediated regulation of in vitro erythropoiesis. It is also suggested that heterogeneous T-cell subsets may exert regulatory functions in the regulation of in vitro hematopoiesis.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V67.4.1143.1143