Structure and expression of the gene encoding phosphofructokinase (PFK) in Drosophila melanogaster

The gene from Drosophila melanogaster that encodes phosphofructokinase has been isolated and its structure and expression characterized. Southern blots of genomic DNAs comparison of multiple clones, and in situ hybridization to salivary gland chromosomes indicate that the Drosophila genome contains...

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Bibliographic Details
Published in:The Journal of biological chemistry 1994-10, Vol.269 (40), p.24679-24687
Main Authors: Currie, P.D. (Syracuse University, Syracuse, NY.), Sullivan, D.T
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
DNA, Complementary - isolation & purification
DROSOPHILA MELANOGASTER
Drosophila melanogaster - enzymology
Drosophila melanogaster - genetics
EXPRESION GENICA
EXPRESSION DES GENES
GENE
Gene Expression
GENES
Molecular Sequence Data
Phosphofructokinase-1 - chemistry
Phosphofructokinase-1 - genetics
RNA, Messenger - analysis
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
TRANSFERASAS
TRANSFERASE
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Summary:The gene from Drosophila melanogaster that encodes phosphofructokinase has been isolated and its structure and expression characterized. Southern blots of genomic DNAs comparison of multiple clones, and in situ hybridization to salivary gland chromosomes indicate that the Drosophila genome contains a single copy of the gene which encodes phosphofructokinase (PFK) that is located at cytogenetic position 46D/E on the polytene map. Analysis of the gene structure reveals a single coding region that spans 6.5 kilobase pairs and is composed of eight exons and seven introns. Three developmentally regulated transcripts are generated through the use of alternative polyadenylation sites. Transcription initiation occurs at a single site. The pattern of transcript accumulation during development is similar to other genes of Drosophila which encode enzymes of the glycolytic pathway. Deficiency analysis of the 46D/E region has identified a lethal complementation group associated with reduced PFK activity, thereby establishing the vital role of PFK function in Drosophila
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)31444-8