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Affinity-purified human interleukin I is cytotoxic to isolated islets of Langerhans

Addition of highly purified human Interleukin-1 to the culture medium of isolated rat islets of Langerhans for 6 days led to 88% inhibition of glucose-induced insulin-release, reduction of islet contents of insulin and glucagon to 31% and 8% respectively, and disintegration of the islets. These effe...

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Bibliographic Details
Published in:Diabetologia 1986, Vol.29 (1), p.63-67
Main Authors: MANDRUP-POULSEN, T, BENDTZEN, K, NERUP, J, DINARELLO, C. A, SVENSON, M, NIELSEN, J. H
Format: Article
Language:English
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Summary:Addition of highly purified human Interleukin-1 to the culture medium of isolated rat islets of Langerhans for 6 days led to 88% inhibition of glucose-induced insulin-release, reduction of islet contents of insulin and glucagon to 31% and 8% respectively, and disintegration of the islets. These effects were dose-dependent and reproducible when using three different Interleukin-1 preparations. Highly purified human Interleukin-2, Lymphotoxin, Leucocyte Migration Inhibitory Factor and Macrophage Migration Inhibitory Factor were ineffective. These findings suggest that Interleukin-1 may play an important role in the molecular mechanisms underlying autoimmune B-cell destruction leading to Type 1 (insulin-dependent) diabetes mellitus.
ISSN:0012-186X
1432-0428
DOI:10.1007/bf02427283