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Segment α 182-198 of Torpedo californica acetylcholine receptor contains a second toxin-binding region and binds anti-receptor antibodies

The area around Cys-192 and Cys-193 is thought to be a functionally important part of the α-subunit of the acetylcholine receptor. We have synthesized peptide α 182-198 of the α-chain of the Torpedo californica acetylcholine receptor and investigated the binding to the peptide of α-bungarotoxin, cob...

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Published in:	FEBS letters 1986-04, Vol.199 (1), p.68-74
Main Authors:	Mulac-Jericevic, Biserka, Atassi, M.Zouhair
Format:	Article
Language:	English
Subjects:	acetylcholine Acetylcholine receptor Amino Acid Sequence Animals Antibody Antigenic site Binding Sites Bungarotoxins - metabolism Cobratoxin Electric Organ - analysis fish physiology Marine neurophysiology neurotransmitters peptides Peptides - chemical synthesis Receptors, Cholinergic - analysis Receptors, Cholinergic - immunology Synthetic peptide Torpedo - metabolism Torpedo californica Toxin-binding region toxins α-Bungarotoxin
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creator	Mulac-Jericevic, Biserka Atassi, M.Zouhair
description	The area around Cys-192 and Cys-193 is thought to be a functionally important part of the α-subunit of the acetylcholine receptor. We have synthesized peptide α 182-198 of the α-chain of the Torpedo californica acetylcholine receptor and investigated the binding to the peptide of α-bungarotoxin, cobratoxin and antibodies raised against acetylcholine receptor. The results showed that the synthetic peptide α 182-198 contains a second toxin-binding region and also binds a considerable fraction of anti-receptor antibodies. We also report here the toxin-binding activity of synthetic peptide a 125–148 of the human acetylcholine receptor which has been previously localized as a toxin-binding region in the α-chain of the Torpedo receptor.
doi_str_mv	10.1016/0014-5793(86)81225-X
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source	ScienceDirect
subjects	acetylcholine Acetylcholine receptor Amino Acid Sequence Animals Antibody Antigenic site Binding Sites Bungarotoxins - metabolism Cobratoxin Electric Organ - analysis fish physiology Marine neurophysiology neurotransmitters peptides Peptides - chemical synthesis Receptors, Cholinergic - analysis Receptors, Cholinergic - immunology Synthetic peptide Torpedo - metabolism Torpedo californica Toxin-binding region toxins α-Bungarotoxin
title	Segment α 182-198 of Torpedo californica acetylcholine receptor contains a second toxin-binding region and binds anti-receptor antibodies
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