Circulating Atrial Natriuretic Peptides in Conscious Rats: Regulation of Release by Multiple Factors

Cardiocytes in the atria contain a prohormone that gives rise to atrial natriuretic peptides (ANP's), which have intrinsic hemodynamic regulatory activity. The distribution of ANP's in the brain suggests the involvement of these peptides in central cardiovascular regulation. In conscious r...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1986-05, Vol.232 (4750), p.636-639
Main Authors: Eskay, Robert, Zukowska-Grojec, Zofia, Haass, Markus, Dave, Jitendra R., Zamir, Nadav
Format: Article
Language:English
Subjects:
Anesthesia
Animals
Antiserum
Atrial Natriuretic Factor - blood
Atrial Natriuretic Factor - isolation & purification
Atrial Natriuretic Factor - metabolism
Atrial Natriuretic Factor - physiology
Biochemistry
Blood
Blood plasma
Blood Volume
Cardiovascular research
Chromatography, High Pressure Liquid
Consciousness - physiology
Elution
Government regulation
Halothane - pharmacology
Heart
Heart - innervation
Heart Atria - drug effects
Heart Atria - metabolism
Heart rate
Hormones
Male
Medical research
Natriuretic peptides
Neurochemistry
Neuroendocrinology
Osmotic Pressure
Pentobarbital - pharmacology
Peptide Fragments - isolation & purification
Peptides
Radioimmunoassay
Rats
Rats, Inbred Strains
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Summary:Cardiocytes in the atria contain a prohormone that gives rise to atrial natriuretic peptides (ANP's), which have intrinsic hemodynamic regulatory activity. The distribution of ANP's in the brain suggests the involvement of these peptides in central cardiovascular regulation. In conscious rats with chronic indwelling catheters, volume loading with isotonic saline or glucose increased the amount of circulating immunoreactive ANP's by a factor of 4 to 5, as determined by radioimmunoassay. Hyperosmotic challenge with a hypertonic NaCl solution or anesthesia with halothane caused similar increases in plasma ANP's. Results obtained with the denervated-heart preparation indicate that neuronal influences are important in the release of ANP's induced by volume loading. As judged from reversed-phase high-performance liquid chromatography of extracted plasma and radioimmunoassay of collected fractions, the circulating physiologically important ANP's in the conscious rodent appear to be α-rANP(5-28) (atriopeptin III) and either α-rANP(3-28) [ANF(8-33)] or α -rANP(1-28) (ANF).
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2938258