Cloning and Analysis of Integrated Hepatitis B Virus DNA of the adr Subtype Derived from a Human Primary Liver Cell Carcinoma

• Published in: Journal of general virology 1986-04, Vol.67 (4), p.771-775
• Main Authors: Fowler, Martin J. F, Thomas, Howard C, Monjardino, John
• Format: Article
• Language: English
• Subjects: Base Sequence; Biological and medical sciences; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - microbiology; Cell Line; Cloning, Molecular; DNA, Viral - genetics; Fundamental and applied biological sciences. Psychology; hepatitis B virus; Hepatitis B virus - genetics; Hepatitis B virus - isolation & purification; Humans; Liver Neoplasms - genetics; Liver Neoplasms - microbiology; Microbiology; Nucleic Acid Hybridization; Recombination, Genetic; Repetitive Sequences, Nucleic Acid; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Virology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-67-4-771

Department of Physiology and 1 Department of Medicine, Royal Free Hospital and School of Medicine, Rowland Hill Street, London NW3 2PF, U.K. A 10 kb genomic DNA fragment derived from a human primary liver cell carcinoma (PLC) and containing integrated hepatitis B virus (HBV) DNA was cloned and analysed. Physical mapping showed the viral DNA to comprise a linear sequence of at least 2·8 kb (87%) of the HBV genome and to be of the adr subtype. Integration appeared to have occurred in the region of the viral genome spanning the cohesive ends. The cellular flanking DNA sequences to one side of the integrated viral DNA contained repeats of the Alu family. The finding of no apparent rearrangements of the integrated HBV DNA sequences in this clone is in contrast to the situation in the huSP and PLC/PRF/5 PLC cell lines in which the integrated viral DNA sequences are greatly rearranged and suggests that such rearrangements may be atypical of solid PLCs. Keywords: HBV, DNA integration, liver cell carcinoma Present address: Molecular Genetics Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 0JG, U.K. Received 23 September 1985; accepted 9 January 1986.