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Molecular identity of a major antigen of Schistosoma mansoni which cross-reacts with Trichinella spiralis and Fasciola hepatica

A glycoprotein cross-reactive among Schistosoma mansoni, Trichinella spiralis and Fasciola hepatica was identified and characterized by use of monoclonal antibodies prepared against S. mansoni glycoproteins. Four monoclonal antibodies recognized the same antigen which was one of the major S. mansoni...

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Published in:Parasitology 1986-02, Vol.92 (1), p.133-151
Main Authors: Aronstein, W. S., Lewis, S. A., Norden, A. P., Dalton, J. P., Strand, M.
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Language:English
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cited_by cdi_FETCH-LOGICAL-c462t-b919b78379d738986261d142af13d2891f9c44061b02b32e817a600d0a26baf73
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description A glycoprotein cross-reactive among Schistosoma mansoni, Trichinella spiralis and Fasciola hepatica was identified and characterized by use of monoclonal antibodies prepared against S. mansoni glycoproteins. Four monoclonal antibodies recognized the same antigen which was one of the major S. mansoni glycoproteins precipitated by sera of hosts infected with either S. mansoni or T. spiralis. This antigen was expressed in S. mansoni cercariae, adult male and female worms, and eggs, and in S. haematobium but not in S. japonicum. Radio-immunoprecipitation and partial proteolytic digest mapping showed that the monoclonal antibodies each recognized a unique epitope. These epitopes were heat labile, sensitive to chaotropic agents, but resistant to reduction and alkylation or digestion with glycosidases, indicating that the recognition sites were amino acids and not carbohydrates. Epitopes recognized by the four monoclonal antibodies were expressed in F. hepatica, whereas only two were expressed in T. spiralis. Analysis by immunofluorescence microscopy showed that the antigen was expressed in S. mansoni in the parenchymal tissue and on the surface of the dorsal tubercles; in mature F. hepatica in the parenchymal tissue, vitelline glands and eggs; in immature F. hepatica only in the parenchymal tissue and in larval T. spiralis in the hypodermis.
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S. ; Lewis, S. A. ; Norden, A. P. ; Dalton, J. P. ; Strand, M.</creator><creatorcontrib>Aronstein, W. S. ; Lewis, S. A. ; Norden, A. P. ; Dalton, J. P. ; Strand, M.</creatorcontrib><description>A glycoprotein cross-reactive among Schistosoma mansoni, Trichinella spiralis and Fasciola hepatica was identified and characterized by use of monoclonal antibodies prepared against S. mansoni glycoproteins. Four monoclonal antibodies recognized the same antigen which was one of the major S. mansoni glycoproteins precipitated by sera of hosts infected with either S. mansoni or T. spiralis. This antigen was expressed in S. mansoni cercariae, adult male and female worms, and eggs, and in S. haematobium but not in S. japonicum. Radio-immunoprecipitation and partial proteolytic digest mapping showed that the monoclonal antibodies each recognized a unique epitope. 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Analysis by immunofluorescence microscopy showed that the antigen was expressed in S. mansoni in the parenchymal tissue and on the surface of the dorsal tubercles; in mature F. hepatica in the parenchymal tissue, vitelline glands and eggs; in immature F. hepatica only in the parenchymal tissue and in larval T. spiralis in the hypodermis.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182000063502</identifier><identifier>PMID: 2421229</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; antigens ; Antigens, Helminth - analysis ; Antigens, Helminth - immunology ; Biological and medical sciences ; cross reaction ; Cross Reactions ; Diseases caused by trematodes ; Epitopes - analysis ; Epitopes - immunology ; Fasciola hepatica ; Fasciola hepatica - immunology ; Female ; glycoproteins ; Glycoproteins - analysis ; Glycoproteins - immunology ; Helminthic diseases ; Infectious diseases ; Male ; Medical sciences ; monoclonal antibodies ; Parasitic diseases ; Schistosoma haematobium - immunology ; Schistosoma japonicum - immunology ; Schistosoma mansoni ; Schistosoma mansoni - growth &amp; development ; Schistosoma mansoni - immunology ; Schistosomiases ; Trichinella - immunology ; Trichinella spiralis ; Tropical medicine</subject><ispartof>Parasitology, 1986-02, Vol.92 (1), p.133-151</ispartof><rights>Copyright © Cambridge University Press 1986</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-b919b78379d738986261d142af13d2891f9c44061b02b32e817a600d0a26baf73</citedby><cites>FETCH-LOGICAL-c462t-b919b78379d738986261d142af13d2891f9c44061b02b32e817a600d0a26baf73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182000063502/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,55689</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=8602663$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2421229$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aronstein, W. S.</creatorcontrib><creatorcontrib>Lewis, S. A.</creatorcontrib><creatorcontrib>Norden, A. P.</creatorcontrib><creatorcontrib>Dalton, J. P.</creatorcontrib><creatorcontrib>Strand, M.</creatorcontrib><title>Molecular identity of a major antigen of Schistosoma mansoni which cross-reacts with Trichinella spiralis and Fasciola hepatica</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>A glycoprotein cross-reactive among Schistosoma mansoni, Trichinella spiralis and Fasciola hepatica was identified and characterized by use of monoclonal antibodies prepared against S. mansoni glycoproteins. Four monoclonal antibodies recognized the same antigen which was one of the major S. mansoni glycoproteins precipitated by sera of hosts infected with either S. mansoni or T. spiralis. This antigen was expressed in S. mansoni cercariae, adult male and female worms, and eggs, and in S. haematobium but not in S. japonicum. 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Analysis by immunofluorescence microscopy showed that the antigen was expressed in S. mansoni in the parenchymal tissue and on the surface of the dorsal tubercles; in mature F. hepatica in the parenchymal tissue, vitelline glands and eggs; in immature F. hepatica only in the parenchymal tissue and in larval T. spiralis in the hypodermis.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>antigens</subject><subject>Antigens, Helminth - analysis</subject><subject>Antigens, Helminth - immunology</subject><subject>Biological and medical sciences</subject><subject>cross reaction</subject><subject>Cross Reactions</subject><subject>Diseases caused by trematodes</subject><subject>Epitopes - analysis</subject><subject>Epitopes - immunology</subject><subject>Fasciola hepatica</subject><subject>Fasciola hepatica - immunology</subject><subject>Female</subject><subject>glycoproteins</subject><subject>Glycoproteins - analysis</subject><subject>Glycoproteins - immunology</subject><subject>Helminthic diseases</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>monoclonal antibodies</subject><subject>Parasitic diseases</subject><subject>Schistosoma haematobium - immunology</subject><subject>Schistosoma japonicum - immunology</subject><subject>Schistosoma mansoni</subject><subject>Schistosoma mansoni - growth &amp; development</subject><subject>Schistosoma mansoni - immunology</subject><subject>Schistosomiases</subject><subject>Trichinella - immunology</subject><subject>Trichinella spiralis</subject><subject>Tropical medicine</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNqFkc1uEzEUhUcIVNLCA7BAeIG6G_C1J_5ZoooUpCJE0krsrDseT-IwMw72RKUrXh1PE2WDBN5YOue7R74-RfEK6DugIN-vKOUAitF8BJ9T9qSYQSV0qUDA02I22eXkPy_OU9o-UoKdFWesYsCYnhW_v4TO2X2HkfjGDaMfH0hoCZIetyESzMraDZO0shufxpBCP5lDCoMn9xtvN8TGkFIZHdoxkXs_bshtzLofXNchSTsfsfMpZzVkgcn6kNWN2-HoLb4onrXYJffyeF8Ud4uPt1efypuv15-vPtyUthJsLGsNupaKS91IrrQSTEADFcMWeMOUhlbbqqICaspqzpwCiYLShiITNbaSXxSXh9xdDD_3Lo2m98lODxxc2CcjhRRqLub_BaGSMAeqMggH8HH96Fqzi77H-GCAmqkd81c7eeb1MXxf9645TRzryP7bo5__Cbs24mB9OmFKUCYEz1h5wHIh7tfJxvjDCMnl3Ijrb6ZaLvVioZbme-bfHPgWg8F1zJF3K0aBU6gqrTXNBD8ug30dfbN2Zhv2cciV_GOdPwW3vkE</recordid><startdate>19860201</startdate><enddate>19860201</enddate><creator>Aronstein, W. 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P. ; Strand, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-b919b78379d738986261d142af13d2891f9c44061b02b32e817a600d0a26baf73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>antigens</topic><topic>Antigens, Helminth - analysis</topic><topic>Antigens, Helminth - immunology</topic><topic>Biological and medical sciences</topic><topic>cross reaction</topic><topic>Cross Reactions</topic><topic>Diseases caused by trematodes</topic><topic>Epitopes - analysis</topic><topic>Epitopes - immunology</topic><topic>Fasciola hepatica</topic><topic>Fasciola hepatica - immunology</topic><topic>Female</topic><topic>glycoproteins</topic><topic>Glycoproteins - analysis</topic><topic>Glycoproteins - immunology</topic><topic>Helminthic diseases</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>monoclonal antibodies</topic><topic>Parasitic diseases</topic><topic>Schistosoma haematobium - immunology</topic><topic>Schistosoma japonicum - immunology</topic><topic>Schistosoma mansoni</topic><topic>Schistosoma mansoni - growth &amp; development</topic><topic>Schistosoma mansoni - immunology</topic><topic>Schistosomiases</topic><topic>Trichinella - immunology</topic><topic>Trichinella spiralis</topic><topic>Tropical medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aronstein, W. S.</creatorcontrib><creatorcontrib>Lewis, S. A.</creatorcontrib><creatorcontrib>Norden, A. P.</creatorcontrib><creatorcontrib>Dalton, J. P.</creatorcontrib><creatorcontrib>Strand, M.</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aronstein, W. S.</au><au>Lewis, S. A.</au><au>Norden, A. P.</au><au>Dalton, J. P.</au><au>Strand, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular identity of a major antigen of Schistosoma mansoni which cross-reacts with Trichinella spiralis and Fasciola hepatica</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>1986-02-01</date><risdate>1986</risdate><volume>92</volume><issue>1</issue><spage>133</spage><epage>151</epage><pages>133-151</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>A glycoprotein cross-reactive among Schistosoma mansoni, Trichinella spiralis and Fasciola hepatica was identified and characterized by use of monoclonal antibodies prepared against S. mansoni glycoproteins. Four monoclonal antibodies recognized the same antigen which was one of the major S. mansoni glycoproteins precipitated by sera of hosts infected with either S. mansoni or T. spiralis. This antigen was expressed in S. mansoni cercariae, adult male and female worms, and eggs, and in S. haematobium but not in S. japonicum. Radio-immunoprecipitation and partial proteolytic digest mapping showed that the monoclonal antibodies each recognized a unique epitope. These epitopes were heat labile, sensitive to chaotropic agents, but resistant to reduction and alkylation or digestion with glycosidases, indicating that the recognition sites were amino acids and not carbohydrates. Epitopes recognized by the four monoclonal antibodies were expressed in F. hepatica, whereas only two were expressed in T. spiralis. Analysis by immunofluorescence microscopy showed that the antigen was expressed in S. mansoni in the parenchymal tissue and on the surface of the dorsal tubercles; in mature F. hepatica in the parenchymal tissue, vitelline glands and eggs; in immature F. hepatica only in the parenchymal tissue and in larval T. spiralis in the hypodermis.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>2421229</pmid><doi>10.1017/S0031182000063502</doi><tpages>19</tpages></addata></record>
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ispartof Parasitology, 1986-02, Vol.92 (1), p.133-151
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source Cambridge University Press:JISC Collections:Full Collection Digital Archives (STM and HSS) (218 titles)
subjects Animals
Antibodies, Monoclonal - immunology
antigens
Antigens, Helminth - analysis
Antigens, Helminth - immunology
Biological and medical sciences
cross reaction
Cross Reactions
Diseases caused by trematodes
Epitopes - analysis
Epitopes - immunology
Fasciola hepatica
Fasciola hepatica - immunology
Female
glycoproteins
Glycoproteins - analysis
Glycoproteins - immunology
Helminthic diseases
Infectious diseases
Male
Medical sciences
monoclonal antibodies
Parasitic diseases
Schistosoma haematobium - immunology
Schistosoma japonicum - immunology
Schistosoma mansoni
Schistosoma mansoni - growth & development
Schistosoma mansoni - immunology
Schistosomiases
Trichinella - immunology
Trichinella spiralis
Tropical medicine
title Molecular identity of a major antigen of Schistosoma mansoni which cross-reacts with Trichinella spiralis and Fasciola hepatica
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