Loading…
Molecular identity of a major antigen of Schistosoma mansoni which cross-reacts with Trichinella spiralis and Fasciola hepatica
A glycoprotein cross-reactive among Schistosoma mansoni, Trichinella spiralis and Fasciola hepatica was identified and characterized by use of monoclonal antibodies prepared against S. mansoni glycoproteins. Four monoclonal antibodies recognized the same antigen which was one of the major S. mansoni...
Saved in:
Published in: | Parasitology 1986-02, Vol.92 (1), p.133-151 |
---|---|
Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c462t-b919b78379d738986261d142af13d2891f9c44061b02b32e817a600d0a26baf73 |
---|---|
cites | cdi_FETCH-LOGICAL-c462t-b919b78379d738986261d142af13d2891f9c44061b02b32e817a600d0a26baf73 |
container_end_page | 151 |
container_issue | 1 |
container_start_page | 133 |
container_title | Parasitology |
container_volume | 92 |
creator | Aronstein, W. S. Lewis, S. A. Norden, A. P. Dalton, J. P. Strand, M. |
description | A glycoprotein cross-reactive among Schistosoma mansoni, Trichinella spiralis and Fasciola hepatica was identified and characterized by use of monoclonal antibodies prepared against S. mansoni glycoproteins. Four monoclonal antibodies recognized the same antigen which was one of the major S. mansoni glycoproteins precipitated by sera of hosts infected with either S. mansoni or T. spiralis. This antigen was expressed in S. mansoni cercariae, adult male and female worms, and eggs, and in S. haematobium but not in S. japonicum. Radio-immunoprecipitation and partial proteolytic digest mapping showed that the monoclonal antibodies each recognized a unique epitope. These epitopes were heat labile, sensitive to chaotropic agents, but resistant to reduction and alkylation or digestion with glycosidases, indicating that the recognition sites were amino acids and not carbohydrates. Epitopes recognized by the four monoclonal antibodies were expressed in F. hepatica, whereas only two were expressed in T. spiralis. Analysis by immunofluorescence microscopy showed that the antigen was expressed in S. mansoni in the parenchymal tissue and on the surface of the dorsal tubercles; in mature F. hepatica in the parenchymal tissue, vitelline glands and eggs; in immature F. hepatica only in the parenchymal tissue and in larval T. spiralis in the hypodermis. |
doi_str_mv | 10.1017/S0031182000063502 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76768565</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cupid>10_1017_S0031182000063502</cupid><sourcerecordid>14715108</sourcerecordid><originalsourceid>FETCH-LOGICAL-c462t-b919b78379d738986261d142af13d2891f9c44061b02b32e817a600d0a26baf73</originalsourceid><addsrcrecordid>eNqFkc1uEzEUhUcIVNLCA7BAeIG6G_C1J_5ZoooUpCJE0krsrDseT-IwMw72RKUrXh1PE2WDBN5YOue7R74-RfEK6DugIN-vKOUAitF8BJ9T9qSYQSV0qUDA02I22eXkPy_OU9o-UoKdFWesYsCYnhW_v4TO2X2HkfjGDaMfH0hoCZIetyESzMraDZO0shufxpBCP5lDCoMn9xtvN8TGkFIZHdoxkXs_bshtzLofXNchSTsfsfMpZzVkgcn6kNWN2-HoLb4onrXYJffyeF8Ud4uPt1efypuv15-vPtyUthJsLGsNupaKS91IrrQSTEADFcMWeMOUhlbbqqICaspqzpwCiYLShiITNbaSXxSXh9xdDD_3Lo2m98lODxxc2CcjhRRqLub_BaGSMAeqMggH8HH96Fqzi77H-GCAmqkd81c7eeb1MXxf9645TRzryP7bo5__Cbs24mB9OmFKUCYEz1h5wHIh7tfJxvjDCMnl3Ijrb6ZaLvVioZbme-bfHPgWg8F1zJF3K0aBU6gqrTXNBD8ug30dfbN2Zhv2cciV_GOdPwW3vkE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14715108</pqid></control><display><type>article</type><title>Molecular identity of a major antigen of Schistosoma mansoni which cross-reacts with Trichinella spiralis and Fasciola hepatica</title><source>Cambridge University Press:JISC Collections:Full Collection Digital Archives (STM and HSS) (218 titles)</source><creator>Aronstein, W. S. ; Lewis, S. A. ; Norden, A. P. ; Dalton, J. P. ; Strand, M.</creator><creatorcontrib>Aronstein, W. S. ; Lewis, S. A. ; Norden, A. P. ; Dalton, J. P. ; Strand, M.</creatorcontrib><description>A glycoprotein cross-reactive among Schistosoma mansoni, Trichinella spiralis and Fasciola hepatica was identified and characterized by use of monoclonal antibodies prepared against S. mansoni glycoproteins. Four monoclonal antibodies recognized the same antigen which was one of the major S. mansoni glycoproteins precipitated by sera of hosts infected with either S. mansoni or T. spiralis. This antigen was expressed in S. mansoni cercariae, adult male and female worms, and eggs, and in S. haematobium but not in S. japonicum. Radio-immunoprecipitation and partial proteolytic digest mapping showed that the monoclonal antibodies each recognized a unique epitope. These epitopes were heat labile, sensitive to chaotropic agents, but resistant to reduction and alkylation or digestion with glycosidases, indicating that the recognition sites were amino acids and not carbohydrates. Epitopes recognized by the four monoclonal antibodies were expressed in F. hepatica, whereas only two were expressed in T. spiralis. Analysis by immunofluorescence microscopy showed that the antigen was expressed in S. mansoni in the parenchymal tissue and on the surface of the dorsal tubercles; in mature F. hepatica in the parenchymal tissue, vitelline glands and eggs; in immature F. hepatica only in the parenchymal tissue and in larval T. spiralis in the hypodermis.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182000063502</identifier><identifier>PMID: 2421229</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; antigens ; Antigens, Helminth - analysis ; Antigens, Helminth - immunology ; Biological and medical sciences ; cross reaction ; Cross Reactions ; Diseases caused by trematodes ; Epitopes - analysis ; Epitopes - immunology ; Fasciola hepatica ; Fasciola hepatica - immunology ; Female ; glycoproteins ; Glycoproteins - analysis ; Glycoproteins - immunology ; Helminthic diseases ; Infectious diseases ; Male ; Medical sciences ; monoclonal antibodies ; Parasitic diseases ; Schistosoma haematobium - immunology ; Schistosoma japonicum - immunology ; Schistosoma mansoni ; Schistosoma mansoni - growth & development ; Schistosoma mansoni - immunology ; Schistosomiases ; Trichinella - immunology ; Trichinella spiralis ; Tropical medicine</subject><ispartof>Parasitology, 1986-02, Vol.92 (1), p.133-151</ispartof><rights>Copyright © Cambridge University Press 1986</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-b919b78379d738986261d142af13d2891f9c44061b02b32e817a600d0a26baf73</citedby><cites>FETCH-LOGICAL-c462t-b919b78379d738986261d142af13d2891f9c44061b02b32e817a600d0a26baf73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182000063502/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,55689</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8602663$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2421229$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aronstein, W. S.</creatorcontrib><creatorcontrib>Lewis, S. A.</creatorcontrib><creatorcontrib>Norden, A. P.</creatorcontrib><creatorcontrib>Dalton, J. P.</creatorcontrib><creatorcontrib>Strand, M.</creatorcontrib><title>Molecular identity of a major antigen of Schistosoma mansoni which cross-reacts with Trichinella spiralis and Fasciola hepatica</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>A glycoprotein cross-reactive among Schistosoma mansoni, Trichinella spiralis and Fasciola hepatica was identified and characterized by use of monoclonal antibodies prepared against S. mansoni glycoproteins. Four monoclonal antibodies recognized the same antigen which was one of the major S. mansoni glycoproteins precipitated by sera of hosts infected with either S. mansoni or T. spiralis. This antigen was expressed in S. mansoni cercariae, adult male and female worms, and eggs, and in S. haematobium but not in S. japonicum. Radio-immunoprecipitation and partial proteolytic digest mapping showed that the monoclonal antibodies each recognized a unique epitope. These epitopes were heat labile, sensitive to chaotropic agents, but resistant to reduction and alkylation or digestion with glycosidases, indicating that the recognition sites were amino acids and not carbohydrates. Epitopes recognized by the four monoclonal antibodies were expressed in F. hepatica, whereas only two were expressed in T. spiralis. Analysis by immunofluorescence microscopy showed that the antigen was expressed in S. mansoni in the parenchymal tissue and on the surface of the dorsal tubercles; in mature F. hepatica in the parenchymal tissue, vitelline glands and eggs; in immature F. hepatica only in the parenchymal tissue and in larval T. spiralis in the hypodermis.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>antigens</subject><subject>Antigens, Helminth - analysis</subject><subject>Antigens, Helminth - immunology</subject><subject>Biological and medical sciences</subject><subject>cross reaction</subject><subject>Cross Reactions</subject><subject>Diseases caused by trematodes</subject><subject>Epitopes - analysis</subject><subject>Epitopes - immunology</subject><subject>Fasciola hepatica</subject><subject>Fasciola hepatica - immunology</subject><subject>Female</subject><subject>glycoproteins</subject><subject>Glycoproteins - analysis</subject><subject>Glycoproteins - immunology</subject><subject>Helminthic diseases</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>monoclonal antibodies</subject><subject>Parasitic diseases</subject><subject>Schistosoma haematobium - immunology</subject><subject>Schistosoma japonicum - immunology</subject><subject>Schistosoma mansoni</subject><subject>Schistosoma mansoni - growth & development</subject><subject>Schistosoma mansoni - immunology</subject><subject>Schistosomiases</subject><subject>Trichinella - immunology</subject><subject>Trichinella spiralis</subject><subject>Tropical medicine</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNqFkc1uEzEUhUcIVNLCA7BAeIG6G_C1J_5ZoooUpCJE0krsrDseT-IwMw72RKUrXh1PE2WDBN5YOue7R74-RfEK6DugIN-vKOUAitF8BJ9T9qSYQSV0qUDA02I22eXkPy_OU9o-UoKdFWesYsCYnhW_v4TO2X2HkfjGDaMfH0hoCZIetyESzMraDZO0shufxpBCP5lDCoMn9xtvN8TGkFIZHdoxkXs_bshtzLofXNchSTsfsfMpZzVkgcn6kNWN2-HoLb4onrXYJffyeF8Ud4uPt1efypuv15-vPtyUthJsLGsNupaKS91IrrQSTEADFcMWeMOUhlbbqqICaspqzpwCiYLShiITNbaSXxSXh9xdDD_3Lo2m98lODxxc2CcjhRRqLub_BaGSMAeqMggH8HH96Fqzi77H-GCAmqkd81c7eeb1MXxf9645TRzryP7bo5__Cbs24mB9OmFKUCYEz1h5wHIh7tfJxvjDCMnl3Ijrb6ZaLvVioZbme-bfHPgWg8F1zJF3K0aBU6gqrTXNBD8ug30dfbN2Zhv2cciV_GOdPwW3vkE</recordid><startdate>19860201</startdate><enddate>19860201</enddate><creator>Aronstein, W. S.</creator><creator>Lewis, S. A.</creator><creator>Norden, A. P.</creator><creator>Dalton, J. P.</creator><creator>Strand, M.</creator><general>Cambridge University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19860201</creationdate><title>Molecular identity of a major antigen of Schistosoma mansoni which cross-reacts with Trichinella spiralis and Fasciola hepatica</title><author>Aronstein, W. S. ; Lewis, S. A. ; Norden, A. P. ; Dalton, J. P. ; Strand, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-b919b78379d738986261d142af13d2891f9c44061b02b32e817a600d0a26baf73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>antigens</topic><topic>Antigens, Helminth - analysis</topic><topic>Antigens, Helminth - immunology</topic><topic>Biological and medical sciences</topic><topic>cross reaction</topic><topic>Cross Reactions</topic><topic>Diseases caused by trematodes</topic><topic>Epitopes - analysis</topic><topic>Epitopes - immunology</topic><topic>Fasciola hepatica</topic><topic>Fasciola hepatica - immunology</topic><topic>Female</topic><topic>glycoproteins</topic><topic>Glycoproteins - analysis</topic><topic>Glycoproteins - immunology</topic><topic>Helminthic diseases</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>monoclonal antibodies</topic><topic>Parasitic diseases</topic><topic>Schistosoma haematobium - immunology</topic><topic>Schistosoma japonicum - immunology</topic><topic>Schistosoma mansoni</topic><topic>Schistosoma mansoni - growth & development</topic><topic>Schistosoma mansoni - immunology</topic><topic>Schistosomiases</topic><topic>Trichinella - immunology</topic><topic>Trichinella spiralis</topic><topic>Tropical medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aronstein, W. S.</creatorcontrib><creatorcontrib>Lewis, S. A.</creatorcontrib><creatorcontrib>Norden, A. P.</creatorcontrib><creatorcontrib>Dalton, J. P.</creatorcontrib><creatorcontrib>Strand, M.</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aronstein, W. S.</au><au>Lewis, S. A.</au><au>Norden, A. P.</au><au>Dalton, J. P.</au><au>Strand, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular identity of a major antigen of Schistosoma mansoni which cross-reacts with Trichinella spiralis and Fasciola hepatica</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>1986-02-01</date><risdate>1986</risdate><volume>92</volume><issue>1</issue><spage>133</spage><epage>151</epage><pages>133-151</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>A glycoprotein cross-reactive among Schistosoma mansoni, Trichinella spiralis and Fasciola hepatica was identified and characterized by use of monoclonal antibodies prepared against S. mansoni glycoproteins. Four monoclonal antibodies recognized the same antigen which was one of the major S. mansoni glycoproteins precipitated by sera of hosts infected with either S. mansoni or T. spiralis. This antigen was expressed in S. mansoni cercariae, adult male and female worms, and eggs, and in S. haematobium but not in S. japonicum. Radio-immunoprecipitation and partial proteolytic digest mapping showed that the monoclonal antibodies each recognized a unique epitope. These epitopes were heat labile, sensitive to chaotropic agents, but resistant to reduction and alkylation or digestion with glycosidases, indicating that the recognition sites were amino acids and not carbohydrates. Epitopes recognized by the four monoclonal antibodies were expressed in F. hepatica, whereas only two were expressed in T. spiralis. Analysis by immunofluorescence microscopy showed that the antigen was expressed in S. mansoni in the parenchymal tissue and on the surface of the dorsal tubercles; in mature F. hepatica in the parenchymal tissue, vitelline glands and eggs; in immature F. hepatica only in the parenchymal tissue and in larval T. spiralis in the hypodermis.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>2421229</pmid><doi>10.1017/S0031182000063502</doi><tpages>19</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0031-1820 |
ispartof | Parasitology, 1986-02, Vol.92 (1), p.133-151 |
issn | 0031-1820 1469-8161 |
language | eng |
recordid | cdi_proquest_miscellaneous_76768565 |
source | Cambridge University Press:JISC Collections:Full Collection Digital Archives (STM and HSS) (218 titles) |
subjects | Animals Antibodies, Monoclonal - immunology antigens Antigens, Helminth - analysis Antigens, Helminth - immunology Biological and medical sciences cross reaction Cross Reactions Diseases caused by trematodes Epitopes - analysis Epitopes - immunology Fasciola hepatica Fasciola hepatica - immunology Female glycoproteins Glycoproteins - analysis Glycoproteins - immunology Helminthic diseases Infectious diseases Male Medical sciences monoclonal antibodies Parasitic diseases Schistosoma haematobium - immunology Schistosoma japonicum - immunology Schistosoma mansoni Schistosoma mansoni - growth & development Schistosoma mansoni - immunology Schistosomiases Trichinella - immunology Trichinella spiralis Tropical medicine |
title | Molecular identity of a major antigen of Schistosoma mansoni which cross-reacts with Trichinella spiralis and Fasciola hepatica |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T05%3A43%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20identity%20of%20a%20major%20antigen%20of%20Schistosoma%20mansoni%20which%20cross-reacts%20with%20Trichinella%20spiralis%20and%20Fasciola%20hepatica&rft.jtitle=Parasitology&rft.au=Aronstein,%20W.%20S.&rft.date=1986-02-01&rft.volume=92&rft.issue=1&rft.spage=133&rft.epage=151&rft.pages=133-151&rft.issn=0031-1820&rft.eissn=1469-8161&rft.coden=PARAAE&rft_id=info:doi/10.1017/S0031182000063502&rft_dat=%3Cproquest_cross%3E14715108%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c462t-b919b78379d738986261d142af13d2891f9c44061b02b32e817a600d0a26baf73%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=14715108&rft_id=info:pmid/2421229&rft_cupid=10_1017_S0031182000063502&rfr_iscdi=true |