Activation of Raf-1 by 14-3-3 proteins

• Published in: Nature (London) 1994-10, Vol.371 (6498), p.612-614
• Main Authors: Fantl, Wendy J, Muslin, Anthony J, Kikuchi, Akira, Martin, Jay A, MacNicol, Angus M, Grosst, Richard W, Williams, Lewis T
• Format: Article
• Language: English
• Subjects: 14-3-3 Proteins; Animals; Biochemistry; Biological and medical sciences; CDC2 Protein Kinase - metabolism; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; Cells, Cultured; Enzyme Activation; Fundamental and applied biological sciences. Psychology; Humanities and Social Sciences; letter; Molecular and cellular biology; multidisciplinary; Mutation; Nerve Tissue Proteins - metabolism; Oocytes; Oogenesis - genetics; Oogenesis - physiology; Protein-Serine-Threonine Kinases - metabolism; Proteins; Proto-Oncogene Proteins - metabolism; Proto-Oncogene Proteins c-raf; Recombinant Proteins - metabolism; Saccharomyces cerevisiae - genetics; Science; Science (multidisciplinary); Tyrosine 3-Monooxygenase; Xenopus; Yeast; Yeasts
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/371612a0

THE protein Raf-1, a key mediator of mitogenesis and differentia-tion, associates with p21*"a5 (refs 1-3). However, the regulation of the serine/threonine kinase activity of Raf-1 is still not understood4 13. Using the yeast two-hybrid system8'14"16, we identi-fied two structurally related proteins that interact with the amino-terminal region of Raf-1. These proteins, 14-3-3 £ (PLA2) and 14-3-3 p (HS1), are members of the 14-3-3 family of proteins17'23. Expression of 14-3-3 proteins in Xenopm oocytes enhanced Raf-1 activity and promoted Raf-1-dependent oocyte maturation. A dominant negative mutant of Raf-1 blocked the effects of 14-3-3 protein.