High-performance liquid chromatography and micellar electrokinetic chromatography of taxol and related taxanes from bark and needle extracts of Taxus species

High-performance liquid chromatography (HPLC) and micellar electrokinetic chromatography (MEKC) were applied for the separation of taxol, cephalomannine, and baccatin III in crude extracts from the needle and bark of Taxus species. The chromatogram of the bark extract was cleaner than that of the ne...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical applications Biomedical applications, 1994-07, Vol.657 (2), p.301-306
Main Authors: Chan, King C., Belinda Alvarado, A., McGuire, Mary T., Muschik, Gary M., Issaq, Haleem J., Snader, Kenneth M.
Format: Article
Language:English
Subjects:
Alkaloids - analysis
Analytical, structural and metabolic biochemistry
Antineoplastic Agents, Phytogenic - analysis
Biological and medical sciences
Chromatography, High Pressure Liquid
Chromatography, Liquid - methods
Drugs, Chinese Herbal - analysis
Fundamental and applied biological sciences. Psychology
Micelles
Other biological molecules
Paclitaxel - analogs & derivatives
Paclitaxel - analysis
Spectrophotometry, Ultraviolet
Taxoids
Terpenes, steroids. Hormones
Trees - chemistry
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Summary:High-performance liquid chromatography (HPLC) and micellar electrokinetic chromatography (MEKC) were applied for the separation of taxol, cephalomannine, and baccatin III in crude extracts from the needle and bark of Taxus species. The chromatogram of the bark extract was cleaner than that of the needle allowing a more reliable detection of taxol and cephalomannine in the bark extract. However, HPLC quantitation of taxol in the needle extract would be difficult due to coeluting taxinines. Nevertheless, this was not a problem in the MEKC experiment. In comparison to HPLC, MEKC offered baseline resolution of taxol from taxinines in the needle extract, less solvent waste, a smaller sample requirement, and the simultaneous detection of taxol, cephalomannine and baccatin III in a relatively simpler electrophoretic run.
ISSN:0378-4347
1572-6495
DOI:10.1016/S0378-4347(94)80006-5