Oxygen dependence of vitamin K-dependent carboxylase and vitamin K epoxidase

A study of the oxygen requirements of the rat liver microsomal vitamin K-dependent carboxylase and vitamin K 2,3-epoxidase indicated that both enzymes had a K m for O 2 in the range 60–80 μM. This value was not influenced by vitamin concentration, alterations in carboxylase substrate, Mn 2+, or dith...

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Bibliographic Details
Published in:FEBS letters 1986-05, Vol.200 (1), p.71-75
Main Authors: McTigue, John J., Suttie, J.W.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Carbon-Carbon Ligases
Dithiothreitol - pharmacology
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Kinetics
Ligases - metabolism
Lyases
Manganese - pharmacology
Microsomes, Liver - enzymology
Mixed Function Oxygenases - metabolism
Oxygen Consumption
Rats
Vitamin K Deficiency - enzymology
Vitamin K Epoxide Reductases
Vitamin K Oxygen Carboxylation Epoxidation γ-Carboxyglutamic acid
Vitamin KOxygenCarboxylationEpoxidationγ-Carboxyglutamic acid
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Summary:A study of the oxygen requirements of the rat liver microsomal vitamin K-dependent carboxylase and vitamin K 2,3-epoxidase indicated that both enzymes had a K m for O 2 in the range 60–80 μM. This value was not influenced by vitamin concentration, alterations in carboxylase substrate, Mn 2+, or dithiothreitol, and is consistent with the hypothesis that both activities are catalyzed by the same enzyme.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(86)80513-0