Crystallographic Study of Cytochrome c553 from Desulfovibrio vulgaris Miyazaki

Cytochrome c553 from the sulfate-reducing bacterium, Desulfovibrio vulgaris Miyazaki, has been crystallized. The combination of microdialysis and vapor diffusion allowed successful crystallization. The crystals were of good quality, and useful data were obtained that extended to the nominal resoluti...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1986, Vol.99 (2), p.605-606
Main Authors: NAKAGAWA, Atsushi, NAGASHIMA, Etsuro, HIGUCHI, Yoshiki, KUSUNOKI, Masami, MATSUURA, Yoshiki, YASUOKA, Noritake, KATSUBE, Yukiteru, CHIHARA, Hideaki, YAGI, Tatsuhiko
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Crystallization
Cytochrome c Group - isolation & purification
Desulfovibrio - analysis
Fundamental and applied biological sciences. Psychology
Microbiology
Morphology, structure, chemical composition
X-Ray Diffraction
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Summary:Cytochrome c553 from the sulfate-reducing bacterium, Desulfovibrio vulgaris Miyazaki, has been crystallized. The combination of microdialysis and vapor diffusion allowed successful crystallization. The crystals were of good quality, and useful data were obtained that extended to the nominal resolution of 1·3 Å. The space group is P43212 with cell dimensions of a= b=42·7 Å, c= 103·4 Å. More than twenty heavyatom reagents were screened with the isomorphous replacement technique, and only the mersalyl derivative could be used for the phase determination. The single isomorphous replacement method combined with the anomalous scattering effect of the Hg-atom in mersalyl and the Fe-atom of the heme group was used for the phase determination.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a135518