Neutralizing Secretory IgA and IgG Do Not Inhibit Attachment of Transmissible Gastroenteritis Virus

INRA, Laboratoire de Pathologie Porcine, 37380 Nouzilly, France Secretory IgA (sIgA) and IgG from porcine milk and serum, respectively, [ 3 H]uridine-labelled virus, swine testis and pig kidney cell lines were used to examine the neutralized virus-cell interaction. Transmissible gastroenteritis viru...

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Bibliographic Details
Published in:Journal of general virology 1986-05, Vol.67 (5), p.939-943
Main Authors: Nguyen, Tien Dung, Bottreau, Elisabeth, Bernard, Serge, Lantier, Isabelle, Aynaud, Jean Marie
Format: Article
Language:English
Subjects:
Animals
Antigen-Antibody Complex
Biological and medical sciences
Cell Line
Coronaviridae - physiology
Female
Fundamental and applied biological sciences. Psychology
Immunoglobulin A - isolation & purification
Immunoglobulin A - physiology
Immunoglobulin G - isolation & purification
Immunoglobulin G - physiology
Kidney
Male
Microbiology
Milk - immunology
Pregnancy
Receptors, Virus - immunology
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Swine
Testis
transmissible gastroenteritis virus
Transmissible gastroenteritis virus - immunology
Transmissible gastroenteritis virus - physiology
Virology
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Summary:INRA, Laboratoire de Pathologie Porcine, 37380 Nouzilly, France Secretory IgA (sIgA) and IgG from porcine milk and serum, respectively, [ 3 H]uridine-labelled virus, swine testis and pig kidney cell lines were used to examine the neutralized virus-cell interaction. Transmissible gastroenteritis virus (TGEV), 99.99% neutralized by immunoglobulin, was able to attach to the cells. Moreover, sIgA enhanced virus attachment. However, the neutralized virus was unable to enter cells, as demonstrated by the action of proteinase K which removed it from the cell surface. It was also found that pre-attached virus was still neutralizable and that IgG and sIgA had similar TGEV-neutralizing capacities. Keywords: TGEV, neutralization, attachment Permanent address: National Institute of Veterinary Research, Bachmai, Hanoi, Vietnam. Received 20 August 1985; accepted 23 January 1986.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-67-5-939