Identification and characterization of genes induced during sexual differentiation in Schizosaccharomyces pombe

Five cDNA clones, harboring genetic messages preferentially expressed during the sexual differentiation process, were isolated from a cDNA library of Schizosaccharomyces pombe by subtractive screening. Transcription of the corresponding genes, termed isp3, 4, 5, 6, and 7, was dependent on nitrogen s...

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Bibliographic Details
Published in:Current genetics 1994-07, Vol.26 (1), p.31-37
Main Authors: SATO, S, SUZUKI, H, WIDYASTUTI, U, HOTTA, Y, TABATA, S
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acid Transport Systems
Biological and medical sciences
Carrier Proteins
Cell Cycle
DNA, Complementary - genetics
DNA, Fungal - genetics
Fundamental and applied biological sciences. Psychology
Fungal Proteins - genetics
Gene Expression Regulation, Fungal
Genes, Fungal
Genes. Genome
Membrane Transport Proteins - genetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Schizosaccharomyces - genetics
Schizosaccharomyces - physiology
Schizosaccharomyces pombe
Schizosaccharomyces pombe Proteins
Sequence Alignment
Sequence Homology, Amino Acid
Serine Endopeptidases - genetics
Sex Differentiation - genetics
Spores, Fungal
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Summary:Five cDNA clones, harboring genetic messages preferentially expressed during the sexual differentiation process, were isolated from a cDNA library of Schizosaccharomyces pombe by subtractive screening. Transcription of the corresponding genes, termed isp3, 4, 5, 6, and 7, was dependent on nitrogen starvation and their induction occurred at several stages of spore formation. Analysis of the cDNA primary structures revealed a capacity for the coding of polypeptides of 19.2 kDa, 88.3 kDa, 60.1 kDa, 49.7 kDa, and 43.8 kDa, respectively. The translated amino-acid sequences of isp5 and isp6 were found to show significant similarities to those of amino-acid permeases and proteinase B of Saccharomyces cerevisiae, respectively. Disruption of isp6 arrested the cell cycle prior to conjugation and caused a drastic blocking effect on spore formation.
ISSN:0172-8083
1432-0983
DOI:10.1007/bf00326301