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Identification and characterization of genes induced during sexual differentiation in Schizosaccharomyces pombe
Five cDNA clones, harboring genetic messages preferentially expressed during the sexual differentiation process, were isolated from a cDNA library of Schizosaccharomyces pombe by subtractive screening. Transcription of the corresponding genes, termed isp3, 4, 5, 6, and 7, was dependent on nitrogen s...
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| Published in: | Current genetics 1994-07, Vol.26 (1), p.31-37 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c449t-e6cf8fb2051bb47410c37c5b7f8db227431c0fb61414e0c30328f928c55e38413 |
| container_end_page | 37 |
| container_issue | 1 |
| container_start_page | 31 |
| container_title | Current genetics |
| container_volume | 26 |
| creator | SATO, S SUZUKI, H WIDYASTUTI, U HOTTA, Y TABATA, S |
| description | Five cDNA clones, harboring genetic messages preferentially expressed during the sexual differentiation process, were isolated from a cDNA library of Schizosaccharomyces pombe by subtractive screening. Transcription of the corresponding genes, termed isp3, 4, 5, 6, and 7, was dependent on nitrogen starvation and their induction occurred at several stages of spore formation. Analysis of the cDNA primary structures revealed a capacity for the coding of polypeptides of 19.2 kDa, 88.3 kDa, 60.1 kDa, 49.7 kDa, and 43.8 kDa, respectively. The translated amino-acid sequences of isp5 and isp6 were found to show significant similarities to those of amino-acid permeases and proteinase B of Saccharomyces cerevisiae, respectively. Disruption of isp6 arrested the cell cycle prior to conjugation and caused a drastic blocking effect on spore formation. |
| doi_str_mv | 10.1007/bf00326301 |
| format | article |
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| identifier | ISSN: 0172-8083 |
| ispartof | Current genetics, 1994-07, Vol.26 (1), p.31-37 |
| issn | 0172-8083 1432-0983 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76809374 |
| source | Springer Archives (Through 1996) |
| subjects | Amino Acid Sequence Amino Acid Transport Systems Biological and medical sciences Carrier Proteins Cell Cycle DNA, Complementary - genetics DNA, Fungal - genetics Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics Gene Expression Regulation, Fungal Genes, Fungal Genes. Genome Membrane Transport Proteins - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Schizosaccharomyces - genetics Schizosaccharomyces - physiology Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins Sequence Alignment Sequence Homology, Amino Acid Serine Endopeptidases - genetics Sex Differentiation - genetics Spores, Fungal |
| title | Identification and characterization of genes induced during sexual differentiation in Schizosaccharomyces pombe |
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