Fate Of Borrelia burgdorferi DNA In Tissues Of Infected Mice After Antibiotic Treatment

Persistence of Borrelia burgdorferi DNA in tissues following antibiotic treatment was evaluated in C3H mice inoculated intradermally with 103 B. burgdorferi N40 or sterile medium. Half of the infected mice and all of the uninfected mice were treated with ceftriaxone 15 days after inoculation for 5 d...

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Bibliographic Details
Published in:The Journal of infectious diseases 1994-11, Vol.170 (5), p.1312-1316
Main Authors: Malawista, Stephen E., Barthold, Stephen W., Persing, David H.
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - therapeutic use
Antibiotics
Antigens, Bacterial
Antigens, Surface - genetics
Bacterial Outer Membrane Proteins - genetics
Bacterial Vaccines
Borrelia burgdorferi
Borrelia burgdorferi Group - genetics
Ceftriaxone - therapeutic use
Ciprofloxacin - therapeutic use
Concise Communications
DNA
DNA, Bacterial - analysis
Female
Infections
Inoculation
Lipoproteins
Lyme disease
Lyme Disease - drug therapy
Lyme Disease - microbiology
Male
Mice
Mice, Inbred C3H
Polymerase Chain Reaction
Spirochaetales
Tissue culture techniques
Urinary bladder
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Summary:Persistence of Borrelia burgdorferi DNA in tissues following antibiotic treatment was evaluated in C3H mice inoculated intradermally with 103 B. burgdorferi N40 or sterile medium. Half of the infected mice and all of the uninfected mice were treated with ceftriaxone 15 days after inoculation for 5 days. Ear and urinary bladder samples were collected on days 20, 30, and 60 after inoculation for culture and for extraction of DNA and amplification of specific spirochetal DNA by polymerase chain reaction (PCR). PCR primers were specific for a 280-bp portion of a highly conserved region ofthe gene encoding outer surface protein (Osp) A of B. burgdorferi and for a 328-bp part of the OspB gene. There was excellent concordance between culture and PCR for ears (35/36 mice) and bladders (33/36). Both tissues became uniformly negative at the earliest interval tested after antibiotic treatment. Thus, the ability to amplify B. burgdorferi DNA quickly disappeared from tissues that had become culture-negative after antibiotic treatment, suggesting that serial study of PCR-positive tissues and fluids may be useful for evaluating the efficacy of antibiotic therapy in human Lyme disease.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/170.5.1312