Detection and Characterization of Antibodies to Bacterial Heat-Shock Protein 60 in Sera of Patients with Primary Biliary Cirrhosis

The enzyme-linked immunosorbent assay (ELISA) with bacterial heat-shock protein 60 (HSP60) purified from Yersinia enterocolitica (Ye) revealed that the antibodies directed against YeHSP60 existed in sera of patients with primary biliary cirrhosis (PBC). To characterize the epitope specificity of the...

Full description

Saved in:
Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1994, Vol.38(6), pp.483-487
Main Authors: Yamaguchi, Hiroyuki, Miura, Hitoshi, Ohsumi, Kazuoki, Ishimi, Norikuni, Taguchi, Haruhiko, Ishiyama, Narihiro, Shiraishi, Yasumori, Yamamoto, Tomoko, Ogata, Sachio
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antibodies, Monoclonal
Bacteria
Base Sequence
Biological and medical sciences
Chaperonin 60 - genetics
Chaperonin 60 - immunology
DNA Primers
Electrophoresis, Polyacrylamide Gel
Epitope mapping
Epitopes - analysis
Gastroenterology. Liver. Pancreas. Abdomen
Heat-shock protein
Humans
Immunoglobulin G - analysis
Liver Cirrhosis, Biliary - blood
Liver Cirrhosis, Biliary - immunology
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Medical sciences
Molecular Sequence Data
Other diseases. Semiology
Polymerase Chain Reaction
Primary biliary cirrhosis
Yersinia enterocolitica
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The enzyme-linked immunosorbent assay (ELISA) with bacterial heat-shock protein 60 (HSP60) purified from Yersinia enterocolitica (Ye) revealed that the antibodies directed against YeHSP60 existed in sera of patients with primary biliary cirrhosis (PBC). To characterize the epitope specificity of the antibodies in patients, the epitope mapping of HSP60 by means of the antibodies was performed. The results have suggested that the epitope recognized with anti-HSP60 antibodies in PBC relates to the amino acid sequence of YeHSP60 molecule as follows: DLGQA-KRVVINKDTTIIIDGVGDEAAIQGRLAQIRQQIEEATSDYDKEK.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1994.tb01813.x