Novel Serine Proteases Encoded by Two Cytotoxic T Lymphocyte-specific Genes

Genes that are expressed exclusively in cytotoxic T cells should encode proteins that are essential for target cell lysis in cell-mediated immune responses. The sequences of two cytotoxic T lymphocyte-specific complementary DNA's (cDNA's) suggest that the two genes encode serine proteases....

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1986-05, Vol.232 (4752), p.858-861
Main Authors: Lobe, Corrinne G., Finlay, B. Brett, Paranchych, William, Paetkau, Verner H., Bleackley, R. Chris
Format: Article
Language:English
Subjects:
Amino acids
Animals
Base Sequence
Binding sites
Biological and medical sciences
Cell lines
Cloning, Molecular
Complementary DNA
Cytotoxic reactions (adcc reaction, cell-mediated lympholysis, complement-dependent cytotoxicity and others)
DNA - genetics
Endopeptidases - genetics
Enzymes
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Genes
Genetic aspects
Genetic research
Genetics
Gin
Immunobiology
Immunological reactions in vitro
Mast cells
Mice
Nucleotides
Numberings
Proteases
Protein research
Proteins
Serine Endopeptidases
Serology
T cells
T lymphocytes
T-Lymphocytes, Cytotoxic - metabolism
Ungulates
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Summary:Genes that are expressed exclusively in cytotoxic T cells should encode proteins that are essential for target cell lysis in cell-mediated immune responses. The sequences of two cytotoxic T lymphocyte-specific complementary DNA's (cDNA's) suggest that the two genes encode serine proteases. A full-length cDNA corresponding to one of the genes was isolated and sequenced. The predicted protein resembles serine proteases in that it includes all the residues that form the catalytic triad of the active site of serine proteases. Moreover, it has sequence characteristics thought to occur only in rat mast cell protease type II. These results are in accord with the view that a protease cascade plays a key role in cytotoxic T-cell activation.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3518058