Growth in semisolid agar of prostate cancer cells obtained from bone marrow aspirates

Thirty-one bone marrow aspirations were performed on patients with prostatic carcinoma metastatic to bone. After separation over a Ficoll-Hypaque gradient viable nucleated cells were cultured in semisolid agar. Colony formation occurred in 14 of 27 (52%) nonbacterially contaminated cultures. Charact...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1986-07, Vol.46 (7), p.3560-3564
Main Authors: AHMANN, F. R, WOO, L, HENDRIX, M, TRENT, J. M
Format: Article
Language:English
Subjects:
Agar
Biological and medical sciences
Biopsy
Bone Marrow - pathology
Bone Neoplasms - pathology
Bone Neoplasms - secondary
Carcinoma - pathology
Cells, Cultured
Humans
Karyotyping
Male
Medical sciences
Microscopy, Electron
Neoplasm Metastasis
Nephrology. Urinary tract diseases
Prostatic Neoplasms - pathology
Tumors of the urinary system
Urinary tract. Prostate gland
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Summary:Thirty-one bone marrow aspirations were performed on patients with prostatic carcinoma metastatic to bone. After separation over a Ficoll-Hypaque gradient viable nucleated cells were cultured in semisolid agar. Colony formation occurred in 14 of 27 (52%) nonbacterially contaminated cultures. Characterization of cells from the colonies showed them to be consistent with malignant prostate cells. After staining, these cells were periodic acid-Schiff positive, prostatic acid phosphatase positive, and prostatic specific antigen positive. Other studies demonstrated the cells to be karyotypically abnormal, ultrastructurally similar to epithelial cells, and capable of secondary colony formation. Three bone marrow aspirate specimens did not have metastatic prostatic carcinoma detected by standard methods but did demonstrate colony formation. However, colony formation was most frequently seen when a radionuclide scan was positive at the aspiration site and when tumor cells were microscopically detectable by Wright staining of a smeared aspirate. The potential utility of colony forming cultures in prostate cancer is discussed. In working with bone marrow aspirates, additional cell separation procedures may be required to calculate and maximize plating efficiencies.
ISSN:0008-5472
1538-7445