Establishment and Characterization of a Human Plasma Cell Myeloma Culture Having a Rearranged Cellular myc Proto-oncogene

Using a serum-free defined medium, we have established a human cell line, NCI-H929, from a malignant effusion occurring in a patient with IgAk myeloma. The cultured cells have the morphologic, ultrastructural, biochemical, immunologic, and cytochemical features of plasma cells. The cells have rearra...

Full description

Saved in:
Bibliographic Details
Published in:Blood 1986-06, Vol.67 (6), p.1542-1549
Main Authors: Gazdar, Adi F., Oie, Herbert K., Kirsch, Ilan R., Hollis, Gregory F.
Format: Article
Language:English
Subjects:
B-Lymphocytes - immunology
Biological and medical sciences
Cell Line
Deoxyribonuclease HindIII
Deoxyribonucleases, Type II Site-Specific
DNA Restriction Enzymes - metabolism
Epitopes - analysis
Female
Gene Expression Regulation
Humans
Immunodeficiencies. Immunoglobulinopathies
Immunoglobulinopathies
Immunoglobulins - genetics
Immunopathology
Karyotyping
Medical sciences
Microscopy, Electron
Middle Aged
Multiple Myeloma - genetics
Multiple Myeloma - ultrastructure
Proto-Oncogenes
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Using a serum-free defined medium, we have established a human cell line, NCI-H929, from a malignant effusion occurring in a patient with IgAk myeloma. The cultured cells have the morphologic, ultrastructural, biochemical, immunologic, and cytochemical features of plasma cells. The cells have rearranged alpha and kappa genes and synthesize and secrete high amounts of IgAk (>80 Mg/106 cells per 24 hours). The cells express surface immunoglobulin (alpha and kappa), the plasma cell antigen PCA-1, the transferrin receptor (T9) and T10 but lack antigens associated with earlier stages of B cell development (HLA-DR, B1, B2, B4, CALLA), as well as other leukocyte-macro-phage antigens and Epstein-Barr virus (EBV) nuclear antigen. Although molecular studies confirm that both the tumor and cultured cells are derived from the same clone of malignant B cells, the tumor cells were predominantly near-diploid, whereas the cultured cells are predominantly near-tetraploid with six copies of chromosome 8, four to six of which have an 8q + abnormality. However, both the tumor and the cultured cells have a rearrangement of the cellular c-myc proto-oncogene (located at 8q24) and express c-myc RNA. Although a modest number of human “plasmacytoid” cell lines have been established, most are lymphoblastoid lines lacking plasma cell features, while others appear to be early secretory cells. In contrast, NCI-H929 is a differentiated, highly secretory human plasma cell line.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V67.6.1542.1542