Different conformations of the 3' termini of initiator and elongator transfer ribonucleic acids. An EPR study

The 3' ends of transfer ribonucleic acids were covalently labeled with a nitroxide spin label. The 3' end of initiator tRNA (tRNAMetf) from Escherichia coli shows different motional behavior than the 3' terminus of elongator tRNAs as monitored by EPR. The line shapes of the EPR spectr...

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Bibliographic Details
Published in:The Journal of biological chemistry 1986-06, Vol.261 (16), p.7253-7256
Main Authors: Pscheidt, R H, Wells, B D
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Electron Spin Resonance Spectroscopy
Fundamental and applied biological sciences. Psychology
Magnesium - pharmacology
Nucleic Acid Conformation
Nucleic acids
Osmolar Concentration
Rna, ribonucleoproteins
RNA, Transfer
Rotation
Spin Labels
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Summary:The 3' ends of transfer ribonucleic acids were covalently labeled with a nitroxide spin label. The 3' end of initiator tRNA (tRNAMetf) from Escherichia coli shows different motional behavior than the 3' terminus of elongator tRNAs as monitored by EPR. The line shapes of the EPR spectra are quite sensitive to the buffer conditions, as shown by measurements in 4 different buffers. The data are consistent with a constrained or folded back 3' terminus in the initiator tRNA as opposed to the freely rotating elongator 3' terminus. The EPR spectra are also sensitive to aggregation of the tRNA.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)38383-7