A simple fractionation of chicken egg yolk yields a protein component that stimulates cell proliferation and differentiation in primary avian tendon cells

An easily prepared and stable protein fraction from chick egg yolk promotes cell division (40 h generation time) and expression of procollagen (60% of total protein synthesis) in primary avian tendon cells in a serum-free medium. The activity of this yolk fraction (YF) is proteinaceous as reflected...

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Bibliographic Details
Published in:In Vitro Cellular & Developmental Biology 1986-05, Vol.22 (5), p.241-246
Main Authors: Martis, M.J, Schwarz, R.I
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
BIRDS
Cell culture techniques
Cell Differentiation - drug effects
CELL DIVISION
Cell Division - drug effects
Cell growth
Cell physiology
Cells, Cultured
Cellular differentiation
Chick Embryo
Collagens
CRECIMIENTO
CROISSANCE
CULTIVO DE TEJIDOS
CULTURE DE TISSUS
Culture Media
Cultured cells
DIFERENCIACION
DIFFERENCIATION
DIFFERENTIATION
DIVISION CELLULAIRE
DIVISION CELULAR
Egg Proteins - isolation & purification
Egg Proteins - pharmacology
Egg yolk
Egg Yolk - analysis
EGG YOLKS
Flasks
Fundamental and applied biological sciences. Psychology
Gels
GROWTH
GROWTH FACTORS
Growth Substances - isolation & purification
Invited Reviews
Molecular and cellular biology
Molecular Weight
OISEAU
PAJAROS
procollagen
Procollagen - biosynthesis
PROTEINAS
PROTEINE
PROTEINS
Responses to growth factors, tumor promotors, other factors
TENDON
TENDONES
TENDONS
Tendons - cytology
Tendons - metabolism
TISSUE CULTURE
Ungulates
VITELLUS
YEMA DE HUEVO
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Description
Summary:An easily prepared and stable protein fraction from chick egg yolk promotes cell division (40 h generation time) and expression of procollagen (60% of total protein synthesis) in primary avian tendon cells in a serum-free medium. The activity of this yolk fraction (YF) is proteinaceous as reflected by its sensitivity to protease treatment. Yolk fraction is resolved into four major components on sodium dodecyl sulfate polyacrylamide gel electrophoresis with apparent molecular weights of 82,70,42,35 (× 10-3). Under nondenaturing conditions, YF runs as a mixture of high molecular weight aggregates on Sephacryl G-200. We postulate that the active part of YF could be the in ovo growth promoter for embryonic chick tendon cells.
ISSN:0883-8364
2327-431X
1475-2689
DOI:10.1007/BF02621225