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Modulation of cytosolic protein kinase C activity by ferricyanide: priming event seems transmembrane redox signalling: A study on transformed C3H/10T1/2 cells in culture
Transformed 3T3/10T1/2 cultured cells incubated with ferricyanide caused a decrease of 2 mM EDTA extractable cytosolic protein kinase C activity in 2 min, whereas 5 or 20 min ferricyanide treatment reverted the enzyme activity to that observed without ferricyanide. The ferricyanide effect in 2 min w...
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Published in: | FEBS letters 1986-05, Vol.200 (2), p.265-270 |
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creator | Malviya, A.N. Anglard, P. |
description | Transformed 3T3/10T1/2 cultured cells incubated with ferricyanide caused a decrease of 2 mM EDTA extractable cytosolic protein kinase C activity in 2 min, whereas 5 or 20 min ferricyanide treatment reverted the enzyme activity to that observed without ferricyanide. The ferricyanide effect in 2 min was abolished by amiloride and sustained by ouabain. Thus, deactivation-activation of cytosolic protein kinase C is attributed to an unknown signal generation during H
+ accumulation coupled with the Na
+/H
+ exchange phase. In this mechanism the priming event concerns the transmembrane redox process shedding H
+ into the cell interior while impermeant ferricyanide acts as a unique electron acceptor. |
doi_str_mv | 10.1016/0014-5793(86)81149-8 |
format | article |
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+ accumulation coupled with the Na
+/H
+ exchange phase. In this mechanism the priming event concerns the transmembrane redox process shedding H
+ into the cell interior while impermeant ferricyanide acts as a unique electron acceptor.</description><subject>Animals</subject><subject>antiport</subject><subject>Antiporters</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - analysis</subject><subject>Cell Membrane - metabolism</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Cytosol - enzymology</subject><subject>Edetic Acid - pharmacology</subject><subject>Ferricyanides - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Molecular and cellular biology</subject><subject>Ouabain - pharmacology</subject><subject>Oxidation-Reduction</subject><subject>Potassium - metabolism</subject><subject>protein kinase C</subject><subject>Protein Kinase C - metabolism</subject><subject>Protein kinase CTumor promotionTransformed cellTransmembrane signalingFerricyanideNa +/H + antiporter</subject><subject>Protons</subject><subject>Responses to growth factors, tumor promotors, other factors</subject><subject>sodium</subject><subject>Sodium - metabolism</subject><subject>transformed cells</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNqFkcFu1DAQhi0EKtvCG4DkA0JwCGvHjmP3UKlaFYpUxKWcLceeVIYkBttZkUfiLXG6qz3CaTSazzO__x-hV5R8oISKLSGUV02r2Dsp3ktKuarkE7ShsmUV40I-RZsT8hydp_SdlF5SdYbOGFl5sUF_vgQ3Dyb7MOHQY7vkkMLgLf4ZQwY_4R9-MgnwDhub_d7nBXcL7iFGbxczeQeXBfWjnx4w7GHKOAGMCedopjTC2JUKOIILv3HyD5MZhoJe4muc8uwWXM4-on2IIzi8Y7dbSu7ptsYWhiHhosDOQ54jvEDPejMkeHmsF-jbx5v73W119_XT5931XWWZELmitWzbGrqucUa2qqbEdapxTtYAtrXAekNB1qyRteS26QTpuah7QhsnqFWSXaC3h73FgV8zpKxHn1Yx5SNhTroVkktG-H9ByoVSgrUF5AfQxpBShF6vjpm4aEr0GqVec9JrTloK_RilXoW8Pu6fu-LN6dExuzJ_c5ybZM3QFxutTydMEt7wRhXs6oBBMW3vIepkPUwWnI9gs3bB_1vHX9aKvFI</recordid><startdate>19860512</startdate><enddate>19860512</enddate><creator>Malviya, A.N.</creator><creator>Anglard, P.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19860512</creationdate><title>Modulation of cytosolic protein kinase C activity by ferricyanide: priming event seems transmembrane redox signalling: A study on transformed C3H/10T1/2 cells in culture</title><author>Malviya, A.N. ; Anglard, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-128772ebb5da879210db95dd82eec7ce3fa1e82358284c5b60f462f015d61c983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>antiport</topic><topic>Antiporters</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - analysis</topic><topic>Cell Membrane - metabolism</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Cytosol - enzymology</topic><topic>Edetic Acid - pharmacology</topic><topic>Ferricyanides - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Molecular and cellular biology</topic><topic>Ouabain - pharmacology</topic><topic>Oxidation-Reduction</topic><topic>Potassium - metabolism</topic><topic>protein kinase C</topic><topic>Protein Kinase C - metabolism</topic><topic>Protein kinase CTumor promotionTransformed cellTransmembrane signalingFerricyanideNa +/H + antiporter</topic><topic>Protons</topic><topic>Responses to growth factors, tumor promotors, other factors</topic><topic>sodium</topic><topic>Sodium - metabolism</topic><topic>transformed cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Malviya, A.N.</creatorcontrib><creatorcontrib>Anglard, P.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Malviya, A.N.</au><au>Anglard, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of cytosolic protein kinase C activity by ferricyanide: priming event seems transmembrane redox signalling: A study on transformed C3H/10T1/2 cells in culture</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1986-05-12</date><risdate>1986</risdate><volume>200</volume><issue>2</issue><spage>265</spage><epage>270</epage><pages>265-270</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>Transformed 3T3/10T1/2 cultured cells incubated with ferricyanide caused a decrease of 2 mM EDTA extractable cytosolic protein kinase C activity in 2 min, whereas 5 or 20 min ferricyanide treatment reverted the enzyme activity to that observed without ferricyanide. The ferricyanide effect in 2 min was abolished by amiloride and sustained by ouabain. Thus, deactivation-activation of cytosolic protein kinase C is attributed to an unknown signal generation during H
+ accumulation coupled with the Na
+/H
+ exchange phase. In this mechanism the priming event concerns the transmembrane redox process shedding H
+ into the cell interior while impermeant ferricyanide acts as a unique electron acceptor.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3011496</pmid><doi>10.1016/0014-5793(86)81149-8</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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source | ScienceDirect (Online service) |
subjects | Animals antiport Antiporters Biological and medical sciences Carrier Proteins - analysis Cell Membrane - metabolism Cell physiology Cells, Cultured Cytosol - enzymology Edetic Acid - pharmacology Ferricyanides - pharmacology Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Molecular and cellular biology Ouabain - pharmacology Oxidation-Reduction Potassium - metabolism protein kinase C Protein Kinase C - metabolism Protein kinase CTumor promotionTransformed cellTransmembrane signalingFerricyanideNa +/H + antiporter Protons Responses to growth factors, tumor promotors, other factors sodium Sodium - metabolism transformed cells |
title | Modulation of cytosolic protein kinase C activity by ferricyanide: priming event seems transmembrane redox signalling: A study on transformed C3H/10T1/2 cells in culture |
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