Electrical Activity and Calcium Channels in Neuroendocrine Cells

Similar to neuronal cells, neuroendocrine cells express voltage-dependent ion channels and fire action potentials. Ca2+ influx through voltage-dependent Ca2+ channels couples changes in membrane potential to Ca(2+)-dependent cellular processes, such as hormone release. Using the patch-clamp techniqu...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 1994-09, Vol.733 (1), p.335-339
Main Authors: SCHERÜBL, HANS, HESCHELER, JÜRGEN, BYCHKOV, ROSTISLAV, CUBER, JEAN CLAUDE, JOHN, MATHIAS, RIECKEN, ERNST-OTTO, WIEDENMANN, BERTRAM
Format: Article
Language:English
Subjects:
Action Potentials - drug effects
Animals
Calcium - metabolism
Calcium Channels - physiology
Cell Line
Electric Conductivity
Insulinoma
Membrane Potentials
Neurons - physiology
Neurosecretory Systems - physiology
Pancreatic Neoplasms
Pituitary Neoplasms
Somatostatin - pharmacology
Thyroid Neoplasms
Tumor Cells, Cultured
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Summary:Similar to neuronal cells, neuroendocrine cells express voltage-dependent ion channels and fire action potentials. Ca2+ influx through voltage-dependent Ca2+ channels couples changes in membrane potential to Ca(2+)-dependent cellular processes, such as hormone release. Using the patch-clamp technique, we studied the spontaneous electrical activity as well as voltage-dependent Ca2+ channels in cholecystokinin-producing pancreatic cells (RIN 1056E cell line), in prolactin-secreting pituitary cells (GH3 cell line), and in calcitonin-secreting cells of the thyroid (rMTC 44-2 cell line). All three cell types displayed spontaneous electrical activity, that is, they spontaneously produced action potentials. RIN 1056E cells, GH3 cells, and rMTC cells exhibited (various types of) voltage-dependent Ca2+ channels that were regulated by various neurotransmitters and hormones, such as somatostatin.
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.1994.tb17283.x