Localisation of hyaluronate (HA) in primary tumors and nude mouse xenografts of human pancreatic carcinomas using a biotinylated HA-binding protein

A biotinylated hyaluronate (HA)-binding protein isolated from bovine cartilage was used to analyze the distribution of HA in nude mouse xenografts derived from human pancreatic adenocarcinoma cell lines as well as in primary human pancreatic adenocarcinomas. The most reproducible results for the loc...

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Bibliographic Details
Published in:Virchows Archiv : an international journal of pathology 1994, Vol.424 (1), p.7-12
Main Authors: Fries, H, Elsässer, H P, Mahlbacher, V, Neumann, K, Kern, H F
Format: Article
Language:English
Subjects:
Adenocarcinoma - chemistry
Animals
Biotin
Carrier Proteins
Cartilage - chemistry
Cattle
Hyaluronan Receptors
Hyaluronic Acid - analysis
Mice
Mice, Nude
Neoplasm Transplantation
Pancreatic Neoplasms - chemistry
Receptors, Cell Surface
Receptors, Lymphocyte Homing
Trypsin
Tumor Cells, Cultured
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Summary:A biotinylated hyaluronate (HA)-binding protein isolated from bovine cartilage was used to analyze the distribution of HA in nude mouse xenografts derived from human pancreatic adenocarcinoma cell lines as well as in primary human pancreatic adenocarcinomas. The most reproducible results for the localisation of HA were obtained using cryostat sections. When the biotinylated HA-binding protein was applied to histological sections of nude mouse xenografts, the specific staining found could be inhibited by preincubating the HA-binding protein with an excess of HA or by hyaluronidase treatment of the tissue before staining. The highest HA concentration was found at the tumor boundaries, while in the central part of the tumor staining was slight or absent. In cryostat sections of primary tumors HA was found predominantly in the connective tissue immediately around tumor cells or at the border between the tumor and normal pancreatic tissue.
ISSN:0945-6317
1432-2307
DOI:10.1007/BF00197386