Isolation and Quantitation of Long-Chain Acyl-Coenzyme A Esters in Brain Tissue by Solid-Phase Extraction

Long-chain acyl-CoA′s are important intermediates in fatty acid oxidation and phospholipid metabolism. For quantitative analysis of brain acyl-CoA′s, and to avoid decomposition due to high brain acyl-CoA hydrolase activity, a fast and efficient analytical method was developed for isolation and deter...

Full description

Saved in:
Bibliographic Details
Published in:Analytical biochemistry 1994-08, Vol.220 (2), p.321-323
Main Authors: Deutsch, J., Grange, E., Rapoport, S.I., Purdon, A.D.
Format: Article
Language:English
Subjects:
Acyl Coenzyme A - analysis
Acyl Coenzyme A - isolation & purification
Animals
Brain Chemistry
Chromatography, High Pressure Liquid - methods
Palmitoyl Coenzyme A - analysis
Palmitoyl Coenzyme A - isolation & purification
Rats
Rats, Sprague-Dawley
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Long-chain acyl-CoA′s are important intermediates in fatty acid oxidation and phospholipid metabolism. For quantitative analysis of brain acyl-CoA′s, and to avoid decomposition due to high brain acyl-CoA hydrolase activity, a fast and efficient analytical method was developed for isolation and determination of acyl-CoA′s. The analysis includes solid-phase extraction by an oligonucleotide purification cartridge and HPLC measurements using a synthetic internal standard. Estimates of concentration in rat brain are oleoyl-CoA (11.0 nmol/g), palmitoyl-CoA (6.0 nmol/g), stearoyl-CoA (4.0 nmol/g), and linoleoyl- and arachidonoyl-CoA (2.0 nmol/g) for a total concentration of 23 nmol/g brain.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1994.1344