Characterization of energetically functional inverted membrane vesicles from Corynebacterium glutamicum

We show that inverted membrane vesicles from Corynebacteriwn glutamicum, a Gram-positive bacterium, are able to generate and maintain an electrochemical gradient of protons in response to the addition of NADH. This result indicates that the respiratory chain is intact and that the vesicles are reaso...

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Bibliographic Details
Published in:FEBS letters 1994-12, Vol.356 (1), p.104-108
Main Authors: Schrempp, S., Bayan, N., Shechter, E.
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
BHI, brain heart infusion
Biological Transport
CCCP, carbonyl cyanide m-chlorophenyl-hydrazone
Cell Membrane - metabolism
Cell Membrane - ultrastructure
Corynebacterium - metabolism
Corynebacterium - ultrastructure
DCCD, dicyclo-hexylcarbo-diimide
Energy Metabolism
Gram-positive bacterium
Hydrogen-Ion Concentration
Membrane potential
Membrane Potentials
Membrane vesicle
PMS, phenazine methosulfate
Protein translocation
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Summary:We show that inverted membrane vesicles from Corynebacteriwn glutamicum, a Gram-positive bacterium, are able to generate and maintain an electrochemical gradient of protons in response to the addition of NADH. This result indicates that the respiratory chain is intact and that the vesicles are reasonably impermeable to protons. These membrane vesicles may be the starting point for in vitro translocation studies of proteins in Gram-positive bacteria.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(94)01245-8