Type II collagen is transiently expressed during avian cardiac valve morphogenesis

We present new evidence of the temporal and spatial expression of type II collagen in the embryonic chick heart during the very early stages of its development. In particular, we emphasize the distribution of its mRNA and protein during valve formation. Type II collagen as well as several other fibr...

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Bibliographic Details
Published in:Developmental dynamics 1994-08, Vol.200 (4), p.294-304
Main Authors: Swiderski, Ruth E., Daniels, Karla J., Jensen, Karen L., Solursh, Michael
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Chick Embryo - metabolism
Collagen - classification
Collagen - genetics
Collagen - metabolism
Collagens
Embryology: invertebrates and vertebrates. Teratology
Embryonic and Fetal Development
Extracellular matrix
Fundamental and applied biological sciences. Psychology
Heart
Heart Valves - embryology
In Situ Hybridization
Molecular Probes - genetics
Molecular Sequence Data
Organogenesis. Fetal development
Organogenesis. Physiological fonctions
Time Factors
Tissue Distribution
Transcription, Genetic
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Summary:We present new evidence of the temporal and spatial expression of type II collagen in the embryonic chick heart during the very early stages of its development. In particular, we emphasize the distribution of its mRNA and protein during valve formation. Type II collagen as well as several other fibrillar collagens (types I, III, and V) are present in stage 18 endocardial cushion mesenchymal cells. At stage 23, α1(II) collagen transcripts and the cognate polypeptide co‐localize in the atrioventricular valves. As development proceeds, the relative abundance of α1(II) collagen transcripts decreases during the stages studied (stages 22 to 45; day 3.5 to day 19) as assayed by RNA blotting of extracts of whole hearts. Type II collagen protein was immunologically undetectable in stage 38 (day 12) hearts, although collagens I, III, and V persisted and localize in the valve regions, in the endothelial lining of the heart, and in the epicardium. In keeping with other observations of type II collagen expression in non‐chondrogenic regions of a variety of vertebrate embryos, the avian heart also exhibits transient type II collagen expression. © 1994 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/aja.1002000404