Concomitant appearance of intrinsic thermotolerance and storage of trehalose in Saccharomyces cerevisiae during early respiratory phase of batch-culture is CIF1-dependent

• Published in: Microbiology (Society for General Microbiology) 1994-10, Vol.140 (10), p.2625-2632
• Main Authors: Attfield, P.V, Kletsas, S, Hazell, B.W
• Format: Article
• Language: English
• Subjects: developmental stages; Glucose - metabolism; Glucosyltransferases - genetics; Glucosyltransferases - metabolism; heat tolerance; Hot Temperature; Saccharomyces cerevisiae; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; stress response; trehalose; Trehalose - metabolism
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/00221287-140-10-2625

Strains of Saccharomyces cerevisiae that exhibit varied capacities for accumulation of trehalose were tested for intrinsic thermotolerance. Yeast that accumulated trehalose rapidly in early respiratory phase showed equally rapid attainment of thermotolerance, whereas a strain unable to accumulate trehalose at this stage of culture showed markedly delayed appearance of thermotolerance. These results were obtained using closely related but nonisogenic diploids and so it is possible that variable factors other than trehalose were responsible for the observed thermotolerance effects. Therefore, a pair of isogenic diploid S. cerevisiae strains was generated to facilitate further testing of whether trehalose functions in intrinsic stress tolerance. Both isogenic strains inherited a partially reverted cif1 phenotype, designated CPR, from the trehalose-deficient progenitor that had been used in construction of the non-isogenic strains. The CPR phenotype permitted growth on glucose but not accumulation of trehalose, indicating that not all cif1-related deficiencies were suppressed in the CPR strains. However, one of the isogenic CPR pair was cif1/cif1 and failed to accumulate trehalose, whilst the other was cif1/CIF1 and was able to accumulate this sugar. The trehalose-proficient strain showed intrinsic stress tolerance whereas the trehalose-deficient strain was sensitive to heat stress during early respiratory growth. These results suggest that one or more functions of CIF1, not operating in the cif1/cif1(CPR) strains, are important for intrinsic thermotolerance of yeast in early respiratory phase. When considering these results with those of others whose work has indicated a role for trehalose in protection of proteins and membranes, it is reasonable to hypothesize that the trehalose deficiency associated with cif1/cif1(CPR) strains could be a key factor in their intrinsic thermosensitivity. However, if this is the case the importance of trehalose, relative to other stress tolerance factor, appears to vary with growth phase and culture status.