Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats

By using a double-affinity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) mus...

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Bibliographic Details
Published in:Cell and tissue research 1986-01, Vol.244 (2), p.265-270
Main Authors: Yoshimura, N, Murachi, T, Heath, R, Kay, J, Jasani, B, Newman, G R
Format: Article
Language:English
Subjects:
Animals
Calpain - analysis
Calpain - immunology
Gold
Male
Muscles - enzymology
Muscles - ultrastructure
Rats
Rats, Inbred Strains
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Summary:By using a double-affinity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the Z-line.
ISSN:0302-766X
1432-0878
DOI:10.1007/BF00219201