Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats

By using a double-affinity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) mus...

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Published in:Cell and tissue research 1986-01, Vol.244 (2), p.265-270
Main Authors: Yoshimura, N, Murachi, T, Heath, R, Kay, J, Jasani, B, Newman, G R
Format: Article
Language:English
Subjects:
Animals
Calpain - analysis
Calpain - immunology
Gold
Male
Muscles - enzymology
Muscles - ultrastructure
Rats
Rats, Inbred Strains
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container_issue 2
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container_title Cell and tissue research
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creator Yoshimura, N
Murachi, T
Heath, R
Kay, J
Jasani, B
Newman, G R
description By using a double-affinity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the Z-line.
doi_str_mv 10.1007/BF00219201
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ispartof Cell and tissue research, 1986-01, Vol.244 (2), p.265-270
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1432-0878
language eng
recordid cdi_proquest_miscellaneous_76893878
source Springer Archives (Through 1996)
subjects Animals
Calpain - analysis
Calpain - immunology
Gold
Male
Muscles - enzymology
Muscles - ultrastructure
Rats
Rats, Inbred Strains
title Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats
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