Effect of duration of depolarisation on contraction of normal and hypertrophied feline ventricular myocytes

Objective: The aim was to test the hypothesis that the prolongation of action potential duration in hypertrophied feline myocytes causes the contractions to be of long duration. Methods: Left ventricular hypertrophy was induced by slow progressive pressure overload after banding the ascending aorta...

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Bibliographic Details
Published in:Cardiovascular research 1994-10, Vol.28 (10), p.1482-1489
Main Authors: Nuss, H Bradley, Houser, Steven R
Format: Article
Language:English
Subjects:
Action Potentials - physiology
Animals
Biological and medical sciences
Cats
Cell Size
Cells, Cultured
contractility
Electrocardiography. Vectocardiography
Electrodiagnosis. Electric activity recording
electrophysiology
excitation-contracrion coupling
hypertrophy
Hypertrophy, Left Ventricular - pathology
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Myocardial Contraction - physiology
Myocardium - pathology
Patch-Clamp Techniques
Time Factors
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Summary:Objective: The aim was to test the hypothesis that the prolongation of action potential duration in hypertrophied feline myocytes causes the contractions to be of long duration. Methods: Left ventricular hypertrophy was induced by slow progressive pressure overload after banding the ascending aorta of young cats. Single myocytes were en/.ymatically dissociated for whole cell patch clamp studies. Cell shortenings were induced by stimulated action potentials (in current clamp mode) and by step depolarisations using voltage clamp to control the duration of depolarisation. Results: Action potential duration measured at 50% repolarisation (0.5 Hz) was significantly longer in hypertrophied myocytes, at 688(SEM 43) ms, n = 25, v 396(17) ms, n = 22, in control myocytes (p
ISSN:0008-6363
1755-3245
DOI:10.1093/cvr/28.10.1482