A Sandwich Cup Method for the Penetration Assay of Antimicrobial Agents through Pseudomonas Exopolysaccharides

We developed new sandwich cup method to assay the penetration of various antimicrobial agents through Pseudomonas exopolysaccharides. Using alginate extracted from mucoid-type Pseudomonas aeruginosa and gellan gum from Pseudomonas elodea, the role of exopolysaccharides as a barrier against drug pene...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1994, Vol.38(8), pp.615-619
Main Authors: Kumon, Hiromi, Tomochika, Ken-ichi, Matunaga, Tosiyuki, Ogawa, Masatoshi, Ohmori, Hiroyuki
Format: Article
Language:English
Subjects:
Alginates
Anti-Bacterial Agents - pharmacokinetics
Anti-Bacterial Agents - pharmacology
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antimicrobial agent
Biological and medical sciences
Biological Assay
Elodea
Exopolysaccharide
Gels - metabolism
Glucuronic Acid
Hexuronic Acids
Medical sciences
Microbial Sensitivity Tests
Pharmacology. Drug treatments
Polysaccharides, Bacterial - drug effects
Polysaccharides, Bacterial - metabolism
Pseudomonas
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
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Summary:We developed new sandwich cup method to assay the penetration of various antimicrobial agents through Pseudomonas exopolysaccharides. Using alginate extracted from mucoid-type Pseudomonas aeruginosa and gellan gum from Pseudomonas elodea, the role of exopolysaccharides as a barrier against drug penetration was examined. The penetration of positively charged hydrophilic drugs such as aminoglycosides and polypeptides was markedly inhibited by the gels tested, but that of β-lactams, quinolones, and macrolides was not inhibited. The penetration of gentamicin was strongly influenced by the gel concentration, the solution to be used, and the presence of Ca2+. These results suggest that the microenvironment at the infection site could greatly influence drug penetration through biofilms in vivo.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1994.tb01831.x