Patch culturing and transfer of human fetal retinal epithelium

Human retinal pigmented epithelium (RPE) can be cultured by removing small patches of this layer from the choroid of the fetal eye. Such RPE patches give rise to healthy, epithelioid monolayers in vitro within 1-2 weeks without contamination from retinal or choroidal cells. The viability and prolife...

Full description

Saved in:
Bibliographic Details
Published in:Graefe's archive for clinical and experimental ophthalmology 1994-10, Vol.232 (10), p.599-607
Main Authors: GOURAS, P, HUIYUN CAO, YAOHUA SHENG, TANABE, T, EFREMOVA, Y, KJELDBYE, H
Format: Article
Language:English
Subjects:
Adult
Aged
Biological and medical sciences
Cell Division
Cell physiology
Cell Polarity
Cell Survival
Cells, Cultured
Culture Techniques - methods
Fetus
Fundamental and applied biological sciences. Psychology
Humans
Middle Aged
Molecular and cellular biology
Phagocytosis
Pigment Epithelium of Eye - embryology
Pigment Epithelium of Eye - ultrastructure
Vision, photoreception
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human retinal pigmented epithelium (RPE) can be cultured by removing small patches of this layer from the choroid of the fetal eye. Such RPE patches give rise to healthy, epithelioid monolayers in vitro within 1-2 weeks without contamination from retinal or choroidal cells. The viability and proliferative capacity of these cultures is independent of the initial polarity of the patch. These RPE monolayers develop apical/basal polarity and a basal lamina and rest on a field of collagen fibers; they are capable of phagocytizing outer segments. A patch can be lifted off a confluent monolayer and transferred to another culture dish without risking the viability of either the old or the new culture. This provides a means of transplanting an organized, polarized patch of human RPE from one place to another.
ISSN:0721-832X
1435-702X
DOI:10.1007/BF00193120