The detection of a common idiotype of anti-DNA antibodies in the sera of patients with monoclonal gammopathies

The sera of 265 patients with monoclonal gammopathies were examined for the presence of a dominant idiotype of the anti-DNA antibody [16/6 idiotype (Id)] and for anti-DNA activity. An enzyme-linked immunosorbent assay (ELISA) with a rabbit anti-16/6 antibody revealed 23 (8.7%) sera that contained in...

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Bibliographic Details
Published in:Journal of clinical immunology 1986-05, Vol.6 (3), p.194-204
Main Authors: SHOENFELD, Y, BEN-YEHUDA, O, SCHWARTZ, R. S, PICK, A. I, NAPARTSTEK, Y, WILNER, Y, FROLICHMAN, R, SCHATTNER, A, LAVIE, G, JOSHUA, H, PINKHAS, J, KENNEDY, R. C
Format: Article
Language:English
Subjects:
Antibodies, Anti-Idiotypic - analysis
Antigen-Antibody Complex - analysis
Biological and medical sciences
DNA - immunology
DNA, Single-Stranded - immunology
Enzyme-Linked Immunosorbent Assay
Humans
Immune Sera
Immunodeficiencies. Immunoglobulinopathies
Immunoglobulin Idiotypes - analysis
Immunoglobulinopathies
Immunopathology
Medical sciences
Paraproteinemias - blood
Paraproteinemias - immunology
Poly I
Poly T
Reference Values
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Summary:The sera of 265 patients with monoclonal gammopathies were examined for the presence of a dominant idiotype of the anti-DNA antibody [16/6 idiotype (Id)] and for anti-DNA activity. An enzyme-linked immunosorbent assay (ELISA) with a rabbit anti-16/6 antibody revealed 23 (8.7%) sera that contained increased concentrations of the idiotype. Seven of the patients had benign monoclonal gammopathy, three multiple myeloma, three Waldenström macroglobulinemia, five essential mixed cryoglobulinemia, and five monoclonal cryoglobulinemia. In 5 of the 23 sera, antinuclear activity was also noted. In 11 of the 16/6 Id-positive sera the anti-nucleic acid antibody reactions were found to be polyspecific, reacting with polydeoxythymidilic acid and polyinosinic acid, in addition to single-stranded DNA and double-stranded DNA. Similar results were achieved with the purified serum monoclonal components. The specificity of the idiotype analysis was demonstrated with an unrelated dominant idiotype of anti-HBsAg antibody. In none of the patients, except one (with essential mixed cryoglobulinemia), was lupus symptomatology noted.
ISSN:0271-9142
1573-2592
DOI:10.1007/BF00918699