A new human leukemia cell 8.2 kDa differentiation factor: Isolation and primary structure

A new 8.2 kDa differentiation factor has been purified to homogeneity from the cultural media of human myelogenous HL-60 leukemia cells induced by retinoic acid. cDNA clones encoding this factor were isolated from a cDNA library prepared from HL-60 differentiated cells and their nucleotide sequence...

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Bibliographic Details
Published in:FEBS letters 1994-12, Vol.356 (2), p.327-329
Main Authors: Kostanyan, Irina A., Astapova, Mariya V., Starovoytova, Elena V., Dranitsina, Svetlana M., Lipkin, Valery M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Cell Differentiation
Cell Line
Chromatography, Ion Exchange
Cloning, Molecular
Differentiation factor
DNA, Complementary
Electrophoresis, Polyacrylamide Gel
HL-60 cell
Humans
Leukemia, Promyelocytic, Acute
Molecular Sequence Data
Molecular Weight
Neoplasm Proteins - biosynthesis
Neoplasm Proteins - chemistry
Neoplasm Proteins - isolation & purification
Oligonucleotide Probes
Retinoic acid
Tumor Cells, Cultured
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Summary:A new 8.2 kDa differentiation factor has been purified to homogeneity from the cultural media of human myelogenous HL-60 leukemia cells induced by retinoic acid. cDNA clones encoding this factor were isolated from a cDNA library prepared from HL-60 differentiated cells and their nucleotide sequence has been determined. The deduced amino acid sequence of the differentiation factor molecule consists of 54 amino acid residues. The protein is shown to be glycosylated. It was shown by Northern blot experiments that the level of poly(A) + RNA with a length of 450 nucleotides was higher in differentiated cells than in non-differentiated cells.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(94)01306-3