ADP ribosyl cyclase activity of a novel bone marrow stromal cell surface molecule, BST-1

Human BST-1, a bone marrow stromal cell surface molecule, is a GPI-anchored protein that facilitates the growth of pre-B cells. The deduced amino acid sequences of human and mouse BST-1 show around 30% homology with those of CD38 and Aplysia ADP ribosyl cyclase. Therefore, like CD38, BST-1 might pos...

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Bibliographic Details
Published in:FEBS letters 1994-12, Vol.356 (2), p.244-248
Main Authors: Hirata, Yuichi, Kimura, Naoki, Sato, Koh, Ohsugi, Yoshiyuki, Takasawa, Shin, Okamoto, Hiroshi, Ishikawa, Jun, Kaisho, Tsuneyasu, Ishihara, Katsuhiko, Hirano, Toshio
Format: Article
Language:English
Subjects:
ADP ribosyl cyclase
ADP-ribosyl Cyclase 1
ADPR, adenosine diphosphate ribose
Animals
Antigens, CD
Antigens, Differentiation - biosynthesis
Antigens, Differentiation - chemistry
Antigens, Differentiation - metabolism
Base Sequence
Bone Marrow - metabolism
Bone Marrow Cells
BST-1
BST-1, bone marrow stromal cell antigen 1
cADPR hydrolase
cADPR, cyclic adenosine diphosphate ribose
Cations, Divalent - pharmacology
CD38
CHO Cells
Cricetinae
DHFR, dihydrofolate reductase
DNA Primers
GPI, glycosyl-phosphatidylinositol
GPI-Linked Proteins
Humans
Hydrogen-Ion Concentration
IP3, inositol 1,4,5-triphosphate
Kinetics
Membrane Glycoproteins - biosynthesis
Membrane Glycoproteins - chemistry
Membrane Glycoproteins - metabolism
Molecular Sequence Data
N-Glycosyl Hydrolases - biosynthesis
N-Glycosyl Hydrolases - chemistry
N-Glycosyl Hydrolases - metabolism
NAD - metabolism
Polymerase Chain Reaction
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
Recombinant Proteins - metabolism
Stromal Cells - metabolism
Transfection
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Summary:Human BST-1, a bone marrow stromal cell surface molecule, is a GPI-anchored protein that facilitates the growth of pre-B cells. The deduced amino acid sequences of human and mouse BST-1 show around 30% homology with those of CD38 and Aplysia ADP ribosyl cyclase. Therefore, like CD38, BST-1 might possess ADP ribosyl cyclase activity. Here, we report the establishment of a stable transformant CHO cell line which secretes truncated human soluble BST-1, and show that purified soluble BST-1 displays both ADP ribosyl cyclase cADPR hydrolase activities.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(94)01279-2