Quantitative Analysis of Ethyltin Compounds in Mammalian Tissue Using HPLC/FAAS

Measurement of total tin and ethyl, tin forms in mammalian tissue is described, using ion-exchange high performance liquid chromatography (HPLC) in tandem with flameless atomic absorption spectrometry (FAAS) for tin-specific detection. All tin forms in whole blood and tissue homogenates were liberat...

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Bibliographic Details
Published in:Journal of analytical toxicology 1986-05, Vol.10 (3), p.93-97
Main Authors: Orren, David K., Braswell, William M., Mushak, Paul
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Chemical and industrial products toxicology. Toxic occupational diseases
Chromatography, High Pressure Liquid - methods
Medical sciences
Metals and various inorganic compounds
Organotin Compounds - analysis
Organotin Compounds - metabolism
Rats
Spectrophotometry, Atomic
Tin - analysis
Toxicology
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Summary:Measurement of total tin and ethyl, tin forms in mammalian tissue is described, using ion-exchange high performance liquid chromatography (HPLC) in tandem with flameless atomic absorption spectrometry (FAAS) for tin-specific detection. All tin forms in whole blood and tissue homogenates were liberated from biological (in vivo) binding by treatment with 6M hydrochloric acid for a period of 4 hr. Ethyln tin species, as the chlorides, were partitioned into chloroform:ethyl acetate (1:1) and analyzed via HPLC using a strong cation exchange column, with fraction collection by s programmable collector and fraction tin measurement by FAAS. Triethyl- and diethyltin were separated and quantitated using 0.167M ammonium citrate in 70:30 methanol:water, while monoethyltin required the use of 0.50M citrate salt in 70:30 water:methanol as mobile phase to effect elution. The difference between total and speciated tin content provides an estimate of remaining tin species, including the inorganic element.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/10.3.93