Immunochemical Similarity of Synthetic α-(1→3)-Branched D-Glucans to Dextran B1375

Anti-dextran B1375 antibodies were raised in rabbits by injecting formalin-killed Leuconostoc mesenteroides strain NRRL B1375, and the anti-dextran serum was used to examine native dextran B1375, and synthetic linear and four α-(1→3)-branched α-(1→6)-D-glucopyranans for similarities. The antiserum r...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1986, Vol.30(3), pp.261-268
Main Authors: Torii, Mitsuo, Yamazoe, Mari, Ogawa, Setsuko, Watabe, Kazuhito, Koshikawa, Tomihiko, Uryu, Toshiyuki, Schuerch, Conrad
Format: Article
Language:English
Subjects:
Antibodies
Antigen-Antibody Complex
Antigenic determinants, haptens, artificial antigens
Antigens
Biological and medical sciences
Dextrans - immunology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Glucans - immunology
Leuconostoc - immunology
Molecular immunology
Precipitin Tests
Structure-Activity Relationship
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Summary:Anti-dextran B1375 antibodies were raised in rabbits by injecting formalin-killed Leuconostoc mesenteroides strain NRRL B1375, and the anti-dextran serum was used to examine native dextran B1375, and synthetic linear and four α-(1→3)-branched α-(1→6)-D-glucopyranans for similarities. The antiserum reacted with the homologous dextran B1375 and also with all the synthetic linear and branched glucans. Precipitation and precipitation-inhibition studis indicated that the antiserum contained at least three groups of antibodies with different specificities, the first specific for linear α-(1→6)-D-glucopyranan structure, the second specific for α-D-glycopyranosyl-(1→3)-branching and the last specific for another, unknown structure present in the dextran B1375 molecule. Two samples of the synthetic branched glucans were shown to be immunochemically the most similar to natural dextran B1375 by inhibition experiments.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1986.tb00941.x