Significance of detection of immune‐complexed 8 kDa hydatid‐specific antigen for immunodiagnosis of hydatidosis

Three micro‐enzyme‐linked immunosorbent assay (micro‐ELISA) systems were developed and evaluated for detection of specific free circulating antigen and circulating immune‐complexes (CICs) of 8 kDa antigen in the sera of patients with hydatidosis. All (100%) the sera of 30 confirmed positive cases of...

Full description

Saved in:
Bibliographic Details
Published in:FEMS immunology and medical microbiology 1994-09, Vol.9 (3), p.231-236
Main Authors: Kanwar, J.R., Kanwar, Rupinder Kaur, Grewal, A.S., Vinayak, V.K.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Helminth - immunology
Antigen-Antibody Complex - blood
Antigens, Helminth - immunology
Antigens, Helminth - isolation & purification
Biological and medical sciences
Chromatography, Affinity
Diseases caused by cestodes
Echinococcoses
Echinococcosis - diagnosis
Echinococcus - immunology
Echinococcus granulosus
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Helminthic diseases
Humans
Hydatidosis
Immunodiagnosis
Infectious diseases
Medical sciences
Monospecific antibodies
Parasitic diseases
Predictive Value of Tests
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Three micro‐enzyme‐linked immunosorbent assay (micro‐ELISA) systems were developed and evaluated for detection of specific free circulating antigen and circulating immune‐complexes (CICs) of 8 kDa antigen in the sera of patients with hydatidosis. All (100%) the sera of 30 confirmed positive cases of hydatidosis had detectable levels of antigen in the acid‐treated sera. However, 23 (77%) and 26 (87%) sera of 30 confirmed cases had free as well as CICs of 8 kDa antigen in the untreated and in the polyethylene glycol (PEG) precipitated sera, respectively. None of the sera from other patients with parasitic infections or viral hepatitis had any detectable levels of 8 kDa antigen in the untreated, acid‐treated or PEG‐precipitated serum samples. The investigations, therefore, suggested that the demonstration of circulating antigen employing monospecific antibodies to affinity purified 8 kDa antigen in acid‐treated sera is more efficient as compared to detection of free circulating antigen of CICs in the untreated or in the PEG‐precipitated sera which could provide a specific immunodiagnostic tool for ongoing hydatid infection.
ISSN:0928-8244
1574-695X
DOI:10.1111/j.1574-695X.1994.tb00498.x