Effects of in vivo administration of 1,25-dihydroxyvitamin D3 on in vitro proliferation of bovine lymphocytes

Continuous infusion of 1,25-dihydroxyvitamin D3 in the bovine elevated 1,25-dihydroxyvitamin D3 in plasma. Plasma 1,25-dihydroxyvitamin D3 remained elevated until infusions were discontinued. Increased 1,25-dihydroxyvitamin D3 was associated with hypercalcemia (11 to 12 mg/dl) that continued for sev...

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Published in:Journal of dairy science 1994-11, Vol.77 (11), p.3324-3330
Main Authors: Hustmyer, F.G. (Indiana University, Indianapolis), Beitz, D.C, Goff, J.P, Nonnecke, B.J, Horst, R.L, Reinhardt, T.A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
BOVIN
CALCIO
Calcitriol - pharmacology
CALCIUM
Calcium - blood
Cattle - immunology
ESTEROLES
EXPERIMENTACION IN VITRO
EXPERIMENTATION IN VITRO
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
GANADO BOVINO
HORMONAS
HORMONE
IMMUNITE CELLULAIRE
Immunobiology
In Vitro Techniques
Infusion Pumps, Implantable - veterinary
INMUNIDAD CELULAR
LECTINA
LECTINE
Lymphocyte Activation - drug effects
Male
Mitogens - pharmacology
Modulation of the immune response (stimulation, suppression)
Parathyroid Hormone - physiology
PEPTIDE
PEPTIDOS
PLASMA SANGUIN
PLASMA SANGUINEO
Statistics as Topic
STEROL
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Summary:Continuous infusion of 1,25-dihydroxyvitamin D3 in the bovine elevated 1,25-dihydroxyvitamin D3 in plasma. Plasma 1,25-dihydroxyvitamin D3 remained elevated until infusions were discontinued. Increased 1,25-dihydroxyvitamin D3 was associated with hypercalcemia (11 to 12 mg/dl) that continued for several days postinfusion. Elevated 1,25-dihydroxyvitamin D3 also was associated with a significant 70% enhancement of lymphocyte proliferation in cells treated with concanavalin A. Cessation of infusion of 1,25-dihydroxy-vitamin D3 resulted in the return of 1,25-dihydroxyvitamin D3 in plasma and lymphocyte proliferative response to preinfusion amounts. Proliferation in response to pokeweed mitogen was significantly inhibited on d 3 of infusions of 1,25-dihydroxyvitamin D3. Administration of parathyroid hormone for 4 d resulted in a sustained hypercalcemia (approximately 13 mg/dl) and a transient 1-d increase in 1,25-dihydroxyvitamin D3 in plasma. Parathyroid hormone treatment generally did not affect the response of bovine lymphocytes to mitogen. In vivo elevation of 1,25-dihydroxyvitamin D3 is either moderately immunostimulating or exerts no effect on mitogen-stimulated proliferation of lymphocytes. These results contrast with the in vitro results suggesting that 1,25-dihydroxyvitamin D3 is only immunosuppressive
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(94)77273-8