Enzymatic characterization of oral and non-oral black-pigmented Bacteroides species

The purpose of this study was to investigate the production of various enzymes by oral and non-oral black-pigmented Bacteroides species using chromogenic substrates. The 19 substrates present in the API ZYM system did not differentiate between B. melaninogenicus, B. denticola, B. loescheii and B. le...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 1986, Vol.52 (2), p.163-171
Main Authors: van Winkelhoff, A J, van Steenbergen, T J, Kippuw, N, de Graaff, J
Format: Article
Language:English
Subjects:
Bacteroides
Bacteroides - classification
Bacteroides - enzymology
Pigmentation
Species Specificity
Substrate Specificity
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Summary:The purpose of this study was to investigate the production of various enzymes by oral and non-oral black-pigmented Bacteroides species using chromogenic substrates. The 19 substrates present in the API ZYM system did not differentiate between B. melaninogenicus, B. denticola, B. loescheii and B. levii. The asaccharolytic black-pigmented Bacteroides species showed each species specific enzyme activity, however, differences were based on one enzyme only as far as B. asaccharolyticus and B. endodontalis are concerned. An extended number of 40 chromogenic substrates were tested in order to find more species specific enzyme. With a set of 20 substrates it appeared to be possible to discriminate between all species tested. The possibility to use enzymes for the identification of black-pigmented Bacteroides is discussed.
ISSN:0003-6072
1572-9699
DOI:10.1007/bf00429320