monoclonal antibody to lateral element proteins in synaptonemal complexes of Lilium longiflorum

To identify synaptonemal complex (SC) proteins in Lilium longiflorum (lily), monoclonal antibodies were generated using mice immunized with isolated pachytene nuclei. While most of the resulting monoclonal antibodies recognized nucleolar or chromatin proteins, one monoclonal antibody (anti-LE) was f...

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Bibliographic Details
Published in:Chromosoma 1994-09, Vol.103 (5), p.357-367
Main Authors: Anderson, L.K, Stack, S.M, Todd, R.J, Ellis, R.P
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Biological and medical sciences
Cell Nucleus - chemistry
Cytogenetics
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
immunocytochemistry
Immunohistochemistry
Lilium longiflorum
Mice
Molecular Weight
monoclonal antibodies
Nuclear Proteins - analysis
Nuclear Proteins - chemistry
Nuclear Proteins - immunology
nucleoproteins
Plant Leaves - chemistry
Plant Roots - chemistry
Plants - chemistry
Pteridophyta, spermatophyta
synaptonemal complex
Synaptonemal Complex - immunology
Vegetals and fungi
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Summary:To identify synaptonemal complex (SC) proteins in Lilium longiflorum (lily), monoclonal antibodies were generated using mice immunized with isolated pachytene nuclei. While most of the resulting monoclonal antibodies recognized nucleolar or chromatin proteins, one monoclonal antibody (anti-LE) was found that binds to lateral elements. Anti-LE bound more to lateral elements of SCs digested with DNase than to lateral elements that had not been digested with DNase. The opposite pattern of labeling was observed using monoclonal antibodies to lily chromatin and nucleolar proteins. These results indicate that anti-LE is specifically recognizing lateral element proteins and not chromatin or nucleolar proteins surrounding the lateral elements. On immunoblots, anti-LE binds to three pachytene nuclear proteins (Mr 60000, 66000 and 70000), two tetrad (early microspore) nuclear proteins (Mr 60000 and 70000), and two root tip nuclear proteins (Mr 52000 and 60000). However, anti-LE does not bind to proteins from leaf nuclei. Of these four tissues, leaf is the only one that does not have actively dividing cells. This observation suggests that at least some SC proteins are related to nuclear proteins from mitotically active cells.
ISSN:0009-5915
1432-0886
DOI:10.1007/BF00417884