Comparison of T-antigen expression in normal, premalignant, and malignant human colonic tissue using lectin and antibody immunohistochemistry

The Thomsen-Friedenreich antigen has been implicated as a cancer-associated antigen in some human organs including the colon. Most previous studies of Thomsen-Friedenreich antigen expression in the colon used peanut agglutinin (PNA) to identify the immunodeterminant in tissues. However, evidence fro...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1986-09, Vol.46 (9), p.4841-4847
Main Authors: YUAN, M, ITZKOWITZ, S. H, BOLAND, C. R, KIM, Y. D, TOMITA, J. T, PALEKAR, A, BENNINGTON, J. L, TRUMP, B. F, KIM, Y. S
Format: Article
Language:English
Subjects:
Adenoma - immunology
Antibodies, Monoclonal
Antigens, Neoplasm - analysis
Antigens, Tumor-Associated, Carbohydrate
Biological and medical sciences
Blood Group Antigens
Cell Differentiation
Colon - immunology
Colonic Neoplasms - immunology
Disaccharides - analysis
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Hyperplasia - immunology
Intestinal Mucosa - immunology
Intestinal Polyps - immunology
Lectins
Medical sciences
Oligosaccharides - immunology
Peanut Agglutinin
Precancerous Conditions - immunology
Rectal Neoplasms - immunology
Rectum - immunology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
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Summary:The Thomsen-Friedenreich antigen has been implicated as a cancer-associated antigen in some human organs including the colon. Most previous studies of Thomsen-Friedenreich antigen expression in the colon used peanut agglutinin (PNA) to identify the immunodeterminant in tissues. However, evidence from other organs suggests that anti-T antibodies have specificities which differ from those of peanut lectin. To elucidate the nature of the T-immunodeterminant in colonic mucosa, we compared staining by PNA to that of a polyclonal (PAb) and monoclonal (MAb) anti-T antibody. PNA demonstrated the best sensitivity (91%) in cancer tissues but the lowest specificity (68%) in normal mucosa. Staining with MAb was only 76% sensitive but 100% specific. Sensitivity and specificity of PAb were intermediate between PNA and MAb. MAb stained fewer adenomatous polyps than either PNA or PAb, but staining appeared to correlate with premalignant features of the polyps. PNA-binding sites were more prevalent than either PAb or MAb in hyperplastic polyps. Cell cytoplasm was stained by both antibodies more often than by PNA. The majority of fetal colonic specimens stained with all three reagents suggesting that Thomsen-Friedenreich antigen may be an oncodevelopmental antigen in human colon. Differences in staining patterns in some tissues may be due to different antigenic specificities among PNA, PAb, and MAb.
ISSN:0008-5472
1538-7445