Photorepair and Excision Repair Removal of UV-induced Pyrimidine Dimers and (6-4) Photoproducts in the Tail Fin of the Medaka, Oryzias latipes

Induction and repair of UV-B induced DNA damage in the tail fin of the Medaka, were examined immunohistochemicaly and by the enzyme-linked immunosorbent assay (ELISA). UV-induced DNA damage was detected only in the outermost layer of epithelial cells and did not differ in fishes having different deg...

Full description

Saved in:
Bibliographic Details
Published in:JOURNAL OF RADIATION RESEARCH 1994, Vol.35 (3), p.139-146
Main Authors: Funayama, T, Mitani, H, Ishigaki, Y, Matsunaga, T, Nikaido, O, Shima, A
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
DNA - radiation effects
DNA Damage
DNA Repair - physiology
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Oryzias
Oryzias latipes
Pyrimidine Dimers - analysis
Ultraviolet Rays
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Induction and repair of UV-B induced DNA damage in the tail fin of the Medaka, were examined immunohistochemicaly and by the enzyme-linked immunosorbent assay (ELISA). UV-induced DNA damage was detected only in the outermost layer of epithelial cells and did not differ in fishes having different degree of melanization. Both pyrimidine dimers and (6-4) photoproducts in the fin cells were removed by excision repair in the dark, the excision of (6-4) photoproducts being about twice as efficient as that of pyrimidine dimers. The rate of excision repair of UV-induced lesions in fin tissue was three to four times that in cultured Medaka cells, OL32. . In the fin cells, reductions in the numbers of pyrimidine dimers and (6-4) photoproducts were seen after treatment with fluorescent light, whereas less reductions of pyrimidine dimers and no reductions of (6-4) photoproducts were observed in OL32 cells. There are two major types of UV-induced DNA damage: primidine dimers and (6-4) photoproducts.
ISSN:0449-3060
1349-9157
DOI:10.1269/jrr.35.139