Release of 5′-Guanosine Monophosphate and Adenine by Brucella abortus and Their Role in the Intracellular Survival of the Bacteria

Brucella abortus releases two components (fractions 3b and 10) that inhibit the myeloperoxidase-H2O2-halide antibacterial system of bovine neutrophils by inhibiting degranulation. Fractions 3b and 10 were analyzed by high-performance liquid chromatography and thin-layer chromotography and compared w...

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Bibliographic Details
Published in:The Journal of infectious diseases 1986-09, Vol.154 (3), p.464-470
Main Authors: Canning, Peter C., Roth, James A., Deyoe, Billy L.
Format: Article
Language:English
Subjects:
Adenine - metabolism
Animals
Antibacterials
Bacteria
Bacteriology
Biological and medical sciences
Brucella abortus
Brucella abortus - metabolism
Brucella abortus - physiology
Brucella abortus - ultrastructure
Cattle
Chemical bases
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Cytochrome c Group - metabolism
Cytochromes
Fundamental and applied biological sciences. Psychology
Guanine Nucleotides - metabolism
Guanosine Monophosphate - metabolism
Ingestion
Microbiology
Microscopy, Electron
Neutrophils
Neutrophils - microbiology
Nucleotides
Original Articles
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Superoxides
Ungulates
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Summary:Brucella abortus releases two components (fractions 3b and 10) that inhibit the myeloperoxidase-H2O2-halide antibacterial system of bovine neutrophils by inhibiting degranulation. Fractions 3b and 10 were analyzed by high-performance liquid chromatography and thin-layer chromotography and compared with nucleotide and base standards. These investigations indicated that fraction 3b coeluted and comigrated with 5′-guanosine monophosphate (GMP), whereas fraction 10 coeluted and comigrated with adenine. We determined the biologic effects of GMP, adenine, and B. abortus fractions 3b and 10 on neutrophil functions. Iodination activity of neutrophils was inhibited to ∼65% of control by GMP (0.04 mg/ml) and by fraction 3b (0.04 mg/ml). Iodination was also suppressed to ∼80% of control by adenine (0.04 mg/ml) and by fraction 10 (0.04 mg/ml). These results suggest that B. abortus produces GMP and adenine and that these substances contribute to the intracellular survival of the bacteria.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/154.3.464