Early Response Cytokines and Innate Immunity: Essential Roles for TNF Receptor 1 and Type I IL-1 Receptor During Escherichia coli Pneumonia in Mice

• Published in: The Journal of immunology (1950) 2001-03, Vol.166 (6), p.4042-4048
• Main Authors: Mizgerd, Joseph P, Spieker, Matt R, Doerschuk, Claire M
• Format: Article
• Language: English
• Subjects: Active Transport, Cell Nucleus - genetics; Active Transport, Cell Nucleus - immunology; Animals; Antigens, CD - genetics; Antigens, CD - physiology; Cell Nucleus - genetics; Cell Nucleus - immunology; Cell Nucleus - metabolism; Chemokine CXCL1; Chemokine CXCL2; chemokine KC; Chemokines - biosynthesis; Chemokines, CXC; Cytokines - biosynthesis; Escherichia coli; Escherichia coli Infections - genetics; Escherichia coli Infections - immunology; Escherichia coli Infections - pathology; Immunity, Innate - genetics; Inflammation - genetics; Inflammation - immunology; interleukin 1 receptors; Leukocyte Count; Lung - immunology; Lung - pathology; Mice; Mice, Inbred C57BL; Mice, Knockout; Neutrophil Infiltration - genetics; Neutrophil Infiltration - immunology; NF-kappa B - metabolism; Pneumonia, Bacterial - genetics; Pneumonia, Bacterial - immunology; Pneumonia, Bacterial - pathology; Receptors, Interleukin-1 - deficiency; Receptors, Interleukin-1 - genetics; Receptors, Interleukin-1 - physiology; Receptors, Tumor Necrosis Factor - deficiency; Receptors, Tumor Necrosis Factor - genetics; Receptors, Tumor Necrosis Factor - physiology; Receptors, Tumor Necrosis Factor, Type I; Time Factors; TNFR1 protein; tumor necrosis factor receptors
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.166.6.4042

The early response cytokines, TNF and IL-1, have overlapping biologic effects that may function to propagate, amplify, and coordinate host responses to microbial challenges. To determine whether signaling from these early response cytokines is essential to orchestrating innate immune responses to intrapulmonary bacteria, the early inflammatory events induced by instillation of Escherichia coli into the lungs were compared in wild-type (WT) mice and mice deficient in both TNF receptor 1 (TNFR1) and the type I IL-1 receptor (IL1R1). Neutrophil emigration and edema accumulation induced by E. coli were significantly compromised by TNFR1/IL1R1 deficiency. Neutrophil numbers in the circulation and within alveolar septae did not differ between WT and TNFR1/IL1R1 mice, suggesting that decreased neutrophil emigration did not result from decreased sequestration or delivery of intravascular neutrophils. The nuclear translocation of NF-kappa B and the expression of the chemokine macrophage inflammatory protein-2 did not differ between WT and TNFR1/IL1R1 lungs. However, the concentration of the chemokine KC was significantly decreased in the bronchoalveolar lavage fluids of TNFR1/IL1R1 mice compared with that in WT mice. Thus, while many of the molecular and cellular responses to E. coli in the lungs did not require signaling by either TNFR1 or IL1R1, early response cytokine signaling was critical to KC expression in the pulmonary air spaces and neutrophil emigration from the alveolar septae.