Strategies for Prokaryotic Expression of Eukaryotic Membrane Proteins

High‐level heterologous expression of integral membrane proteins at full‐length is a useful tool for their structural and functional characterization. Here, systems that have previously been used for efficient bacterial expression of eukaryotic membrane proteins are reviewed and novel vectors consis...

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Bibliographic Details
Published in:Traffic (Copenhagen, Denmark) Denmark), 2001-01, Vol.2 (2), p.99-104
Main Authors: Laage, Rico, Langosch, Dieter
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Bacteria - genetics
Bacteria - metabolism
Base Sequence
E. coli
Expression
Gene Expression
Genetic Techniques
Genetic Vectors
Immunoblotting
Membrane Proteins - biosynthesis
Membrane Proteins - genetics
Molecular Sequence Data
R-SNARE Proteins
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - genetics
SNARE
SNARE Proteins
synaptobrevin
syntaxin
transmembrane segment
Vesicular Transport Proteins
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Description
Summary:High‐level heterologous expression of integral membrane proteins at full‐length is a useful tool for their structural and functional characterization. Here, systems that have previously been used for efficient bacterial expression of eukaryotic membrane proteins are reviewed and novel vectors consisting of a modular fusion moiety based on nuclease A from Staphylococcus aureus are presented.
ISSN:1398-9219
1600-0854
DOI:10.1034/j.1600-0854.2001.020204.x