Relationships of Microsporidian Genera, with Emphasis on the Polysporous Genera, Revealed by Sequences of the Largest Subunit of RNA Polymerase II (RPB1)

Molecular data have proved useful as an alternative to morphological data in showing the relationships of genera within the phylum Microsporidia, but until now have been available only for ribosomal genes. In previous studies protein-coding genes of microsporidia have been used only to assess their...

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Bibliographic Details
Published in:The Journal of eukaryotic microbiology 2001-01, Vol.48 (1), p.111-117
Main Authors: CHENEY, SARAH A, LAFRANCHI-TRISTEM, NATHALIE J, BOURGES, DOROTHEE, CANNING, ELIZABETH U
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
DNA, Protozoan - analysis
Fish
Fishes - parasitology
Fundamental and applied biological sciences. Psychology
Humans
Insecta - parasitology
insects
man
microsporidia
Microsporidia - classification
Microsporidia - enzymology
Microsporidia - genetics
Microsporidiosis - parasitology
Molecular Sequence Data
Phylogeny
Pleistophora
Protozoa
RNA Polymerase II - genetics
RPB1 gene
Sequence Analysis, DNA
Systematics. Geographical distribution. Morphology. Cytology
Trachipleistophora
Vavraia
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Summary:Molecular data have proved useful as an alternative to morphological data in showing the relationships of genera within the phylum Microsporidia, but until now have been available only for ribosomal genes. In previous studies protein-coding genes of microsporidia have been used only to assess their position in the evolution of eukaryotes. For the first time we report on the use of a protein-coding gene, the A-G region of the largest subunit of RNA polymerase II (RPB1) from 14 mainly polysporous species, to generate an alternative phylogeny for microsporidia. Using the amino acid sequences, the genera and species fell into the same main groupings as had been obtained with 16S rDNA sequences, but the RPB1 data provided better resolution within these groups. The results supported the pairings of Trachipleistophora hominis with Vavraia culicis and Pleistophora hippoglossoideos with Pleistophora typicalis. They also confirmed that the genus Pleistophora is not monophyletic and that it will be necessary to transfer Pleistophora ovariae and Pleistophora mirandellae into one or more other genera, as has already been effected for Pleistophora anguillarum.
ISSN:1066-5234
1550-7408
DOI:10.1111/j.1550-7408.2001.tb00422.x