Improved plasmid vectors for the production of multiple fluorescent protein fusions in Bacillus subtilis

The intrinsically fluorescent green fluorescent protein has been used in many laboratories as a cytological marker to monitor protein localisation in live cells. Multiple spectrally modified mutant versions and novel fluorescent proteins from other species have subsequently been reported and used fo...

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Bibliographic Details
Published in:Gene 2001-02, Vol.264 (2), p.289-297
Main Authors: Feucht, A, Lewis, P J
Format: Article
Language:English
Subjects:
Bacillus subtilis
Bacillus subtilis - genetics
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Cytoskeletal Proteins
DNA, Recombinant
Genetic Vectors - genetics
Green Fluorescent Proteins
Luminescent Proteins - genetics
Luminescent Proteins - metabolism
Microscopy, Fluorescence
Plasmids - genetics
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
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Summary:The intrinsically fluorescent green fluorescent protein has been used in many laboratories as a cytological marker to monitor protein localisation in live cells. Multiple spectrally modified mutant versions and novel fluorescent proteins from other species have subsequently been reported and used for labelling cells with multiple fluorescent protein fusions. In this work we report the design and use of vectors containing some of these spectral variants of GFP for use in the Gram positive bacterium Bacillus subtilis. These vectors complement those previously described (Lewis and Marston, 1999. Gene 227, 101-109) to provide a large suite of plasmid vectors for use in this and other related Gram positive organisms. Using these vectors we have been able to directly demonstrate the sequential assembly/disassembly of proteins involved in the generation of cellular asymmetry during development.
ISSN:0378-1119
DOI:10.1016/s0378-1119(01)00338-9